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Title: Infectious human papillomavirus virions in semen reduce clinical pregnancy rates in women undergoing intrauterine insemination
Authors: Depuydt, Christophe Eric
Donders, Gilbert Ghislain Gerard
Verstraete, Ludo
Vanden Broeck, Davy
Beert, Johan Frans Andre
Salembier, Geert
Bosmans, Eugene
OMBELET, Willem 
Issue Date: 2019
Source: FERTILITY AND STERILITY, 111(6), p. 1135-1144
Abstract: Objective: To study the influence of human papillomavirus (HPV) virions present in different sperm fractions of male partners of women undergoing IUI on fertility outcome. Design: Prospective noninterventional multicenter study. Setting: Inpatient hospital fertility centers. Patient(s): Seven hundred thirty-two infertile couples undergoing 1,753 IUI cycles with capacitated sperm. Intervention(s): None. Main Outcome Measure(s): Biochemical and clinical pregnancy rate in IUI cycles with HPV-positive or HPV-negative semen. Result(s): Five hundred seventy-three infertile couples undergoing 1,362 IUI cycles were enrolled. Work-up of the 1,362 sperm samples that were used for IUI generated 3,444 separate sperm fractions. Each of the sperm fractions was tested with quantitative polymerase chain reaction for 18 different HPV types (6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 67, and 68). HPV prevalence in sperm was 12.5%/IUI cycle. When infectious HPV virions were detected in sperm, a significant decrease in clinical pregnancies was observed when compared with HPV-negative cycles (2.9% vs. 11.1 %/cycle). Above a ratio of 0.66 HPV virions/spermatozoon no pregnancies occurred (sensitivity 100%, specificity 32.5%). Conclusion(s): Women inseminated with HPV-positive sperm had 4 times fewer clinical pregnancies compared with women who had HPV-negative partners. Detection of HPV virions in sperm is associated with a negative IUI outcome and should be part of routine examination and counseling of infertile couples. ((C) 2019 by American Society for Reproductive Medicine.)
Notes: [Depuydt, Christophe Eric; Verstraete, Ludo; Beert, Johan Frans Andre; Bosmans, Eugene] Sonic Healthcare Antwerp, AML, Dept Hormonol & Reprod Hlth, Antwerp, Belgium. [Depuydt, Christophe Eric; Verstraete, Ludo; Beert, Johan Frans Andre; Bosmans, Eugene] Sonic Healthcare, AML, Intermediate Struct Human Body Mat, Antwerp, Belgium. [Donders, Gilbert Ghislain Gerard] Clin Res Women Reg Hosp Heilig Hart, Femicare, Tienen, Belgium. [Donders, Gilbert Ghislain Gerard] Reg Hosp Heilig Hart, Dept Obstet & Gynecol, Tienen, Belgium. [Donders, Gilbert Ghislain Gerard] Univ Hosp Antwerpen, Antwerp, Belgium. [Vanden Broeck, Davy; Beert, Johan Frans Andre; Salembier, Geert] Sonic Healthcare, AML, Dept Clin & Mol Pathol, Antwerp, Belgium. [Vanden Broeck, Davy] Natl Reference Ctr HPV, Brussels, Belgium. [Vanden Broeck, Davy] Univ Ghent, Int Ctr Reprod Hlth, Dept Obstet & Gynecol, Ghent, Belgium. [Ombelet, Willem] ZOL Hosp, Genk Inst Fertil Technol, Genk, Belgium. [Ombelet, Willem] Hasselt Univ, Fac Med & Life Sci, Hasselt, Belgium.
Keywords: Real-time quantitative PCR;HPV;IUI;sperm analysis
Document URI:
ISSN: 0015-0282
e-ISSN: 1556-5653
DOI: 10.1016/j.fertnstert.2019.02.002
ISI #: 000469444000023
Rights: 2019 American Society for Reproductive Medicine, Published by Elsevier Inc
Category: A1
Type: Journal Contribution
Validations: ecoom 2020
Appears in Collections:Research publications

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