Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/13164
Title: Rapid assessment of the stability of DNA duplexes by impedimetric real-time monitoring of chemically induced denaturation
Authors: VAN GRINSVEN, Bart 
VANDEN BON, Natalie 
GRIETEN, Lars 
MURIB, Mohammed Sharif 
JANSSENS, Stoffel 
HAENEN, Ken 
Schneider, E.
Ingebrandt, S.
Schöning, M.J.
VERMEEREN, Veronique 
AMELOOT, Marcel 
MICHIELS, Luc 
THOELEN, Ronald 
DE CEUNINCK, Ward 
WAGNER, Patrick 
Issue Date: 2011
Source: LAB ON A CHIP, 2011(9), p. 1656-1663
Abstract: In this article, we report on the electronic monitoring of DNA denaturation by NaOH using electrochemical impedance spectroscopy in combination with fluorescence imaging as a reference technique. The probe DNA consisting of a 36-mer fragment was covalently immobilized on nanocrystalline-diamond electrodes and hybridized with different types of 29-mer target DNA (complementary, single-nucleotide defects at two different positions, and a non-complementary random sequence). The mathematical separation of the impedimetric signals into the time constant for NaOH exposure and the intrinsic denaturation-time constants gives clear evidence that the denaturation times reflect the intrinsic stability of the DNA duplexes. The intrinsic time constants correlate with calculated DNA-melting temperatures. The impedimetric method requires minimal instrumentation, is label-free and fast with a typical time scale of minutes and is highly reproducible. The sensor electrodes can be used repetitively. These elements suggest that the monitoring of chemically induced denaturation at room temperature is an interesting approach to measure DNA duplex stability as an alternative to thermal denaturation at elevated temperatures, used in DNA-melting experiments and single nucleotide polymorphism (SNP) analysis.
Document URI: http://hdl.handle.net/1942/13164
ISSN: 1473-0197
e-ISSN: 1473-0189
DOI: 10.1039/C1LC20027E
ISI #: 000289363800012
Rights: This journal is © The Royal Society of Chemistry 2011
Category: A1
Type: Journal Contribution
Validations: ecoom 2012
Appears in Collections:Research publications

Show full item record

SCOPUSTM   
Citations

29
checked on Sep 3, 2020

WEB OF SCIENCETM
Citations

31
checked on May 20, 2022

Page view(s)

56
checked on May 26, 2022

Download(s)

44
checked on May 26, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.