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http://hdl.handle.net/1942/14195
Title: | Exploring and comparing the angiogenic properties of different dental stem cell populations | Authors: | FANTON, Yanick | Advisors: | LAMBRICHTS, Ivo BRONCKAERS, Annelies |
Issue Date: | 2012 | Publisher: | tUL Diepenbeek | Abstract: | Therapeutic angiogenesis is very promising, however, current revascularization strategies are unsuccessful. Since angiogenesis is key to the development of the tooth, this study compared the angiogenic potential of 3 dental stem cell (DSC) types: dental pulp stem cells (DPSCs), dental follicle stem cells (FSCs) and stem cells from the apical papilla (SCAPs). Angiogenic factors secreted by the DSCs were identified in a Human Angiogenesis Array, followed by validation by means of ELISA and RT-PCR. In vitro assays with a Human Microvascular Endothelial Cell line (HMEC-1) were performed to gain more insights in the effect of the DSCs on HMEC-1 proliferation, migration and tube formation in an MTT assay, a transwell system and a Matrigel assay respectively. The various DSCs express pro- and anti-angiogenic factors and induce HMEC-1 migration and tube formation, but not proliferation. DSCs thus provide opportunities to treat patients with cells from their own or other individuals' teeth. | Notes: | master in de biomedische wetenschappen-klinische moleculaire wetenschappen | Document URI: | http://hdl.handle.net/1942/14195 | Category: | T2 | Type: | Theses and Dissertations |
Appears in Collections: | Master theses |
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07253842011249.pdf | 3.84 MB | Adobe PDF | View/Open |
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