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DC Field | Value | Language |
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dc.contributor.author | STEVENS, An-Sofie | - |
dc.contributor.author | PIROTTE, Nicky | - |
dc.contributor.author | PLUSQUIN, Michelle | - |
dc.contributor.author | WILLEMS, Maxime | - |
dc.contributor.author | NEYENS, Thomas | - |
dc.contributor.author | ARTOIS, Tom | - |
dc.contributor.author | SMEETS, Karen | - |
dc.date.accessioned | 2014-11-19T13:32:02Z | - |
dc.date.available | 2014-11-19T13:32:02Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | JOURNAL OF APPLIED TOXICOLOGY, 35 (3), p. 319-326 | - |
dc.identifier.issn | 0260-437X | - |
dc.identifier.uri | http://hdl.handle.net/1942/17802 | - |
dc.description.abstract | To investigate hydrophobic test compounds in toxicological studies, solvents like dimethylsulfoxide (DMSO) are inevitable. However, using these solvents, the interpretation of test compound-induced responses can be biased. DMSO concentration guidelines are available, but are mostly based on acute exposures involving one specific toxicity endpoint. Hence, to avoid solvent–toxicant interference, we use multiple chronic test endpoints for additional interpretation of DMSO concentrations and propose a statistical model to assess possible synergistic, antagonistic or additive effects of test compounds and their solvents. In this study, the effects of both short- (1 day) and long-term (2 weeks) exposures to low DMSO concentrations (up to 1000 µl l−1) were studied in the planarian Schmidtea mediterranea. We measured different biological levels in both fully developed and developing animals. In a long-term exposure set-up, a concentration of 500 µl l−1 DMSO interfered with processes on different biological levels, e.g. behaviour, stem cell proliferation and gene expression profiles. After short exposure times, 500 µl l−1 DMSO only affected motility, whereas the most significant changes on different parameters were observed at a concentration of 1000 µl l−1 DMSO. As small sensitivity differences exist between biological levels and developmental stages, we advise the use of this solvent in concentrations below 500 µl l−1 in this organism. In the second part of our study, we propose a statistical approach to account for solvent–toxicant interactions and discuss full-scale solvent toxicity studies. In conclusion, we reassessed DMSO concentration limits for different experimental endpoints in the planarian S. mediterranea. Copyright © 2014 John Wiley & Sons, Ltd. | - |
dc.description.sponsorship | This work was supported by a PhD grant for An-Sofie Stevens from IWT (Agentschap voor Innovatie door Wetenschap en Technologie) (no. 101442). Experiments were financed by Hasselt University BOF-financing (Bijzonder Onderzoeksfonds: BOF08G01) and Hasselt University tUL-impulsfinancing (IMPF2PR). Maxime Willems was funded by an OZM grant by IWT (no. 100631). | - |
dc.language.iso | en | - |
dc.rights | Copyright © 2014 John Wiley & Sons, Ltd. | - |
dc.subject.other | planarians; DMSO; toxicology; different biological levels; solvent–toxicant interference | - |
dc.title | Toxicity profiles and solvent-toxicant interference in the planarian Schmidtea mediterranea after dimethylsulfoxide (DMSO) exposure | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 326 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 319 | - |
dc.identifier.volume | 35 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Correspondence to: Karen Smeets, Zoology: Biodiversity and Toxicology, Centre for Environmental Sciences, Hasselt University, Agoralaan, building D, BE 3590 Diepenbeek, Belgium. karen.smeets@uhasselt.be E-mail: karen.smeets@uhasselt.be | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1002/jat.3011 | - |
dc.identifier.isi | 000349370600012 | - |
item.validation | ecoom 2016 | - |
item.contributor | STEVENS, An-Sofie | - |
item.contributor | PIROTTE, Nicky | - |
item.contributor | PLUSQUIN, Michelle | - |
item.contributor | WILLEMS, Maxime | - |
item.contributor | NEYENS, Thomas | - |
item.contributor | ARTOIS, Tom | - |
item.contributor | SMEETS, Karen | - |
item.accessRights | Restricted Access | - |
item.fullcitation | STEVENS, An-Sofie; PIROTTE, Nicky; PLUSQUIN, Michelle; WILLEMS, Maxime; NEYENS, Thomas; ARTOIS, Tom & SMEETS, Karen (2014) Toxicity profiles and solvent-toxicant interference in the planarian Schmidtea mediterranea after dimethylsulfoxide (DMSO) exposure. In: JOURNAL OF APPLIED TOXICOLOGY, 35 (3), p. 319-326. | - |
item.fulltext | With Fulltext | - |
crisitem.journal.issn | 0260-437X | - |
crisitem.journal.eissn | 1099-1263 | - |
Appears in Collections: | Research publications |
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jat3011.pdf Restricted Access | Published version | 668.36 kB | Adobe PDF | View/Open Request a copy |
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