Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/2319
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | SMETS, Ilse | - |
dc.contributor.author | CAPLANUSI, Adrian | - |
dc.contributor.author | Despa, S | - |
dc.contributor.author | MOLNAR, Zsolt | - |
dc.contributor.author | RADU, Mihai | - |
dc.contributor.author | VAN DE VEN, Martin | - |
dc.contributor.author | AMELOOT, Marcel | - |
dc.contributor.author | STEELS, Paul | - |
dc.date.accessioned | 2007-11-13T15:51:20Z | - |
dc.date.available | 2007-11-13T15:51:20Z | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 286(4). p. F784-F794 | - |
dc.identifier.issn | 0363-6127 | - |
dc.identifier.uri | http://hdl.handle.net/1942/2319 | - |
dc.description.abstract | In ischemic or hypoxic tissues, elevated Ca2+ levels have emerged as one of the main damaging agents among other Ca2+-independent mechanisms of cellular injury. Because mitochondria, besides the endoplasmic reticulum, play a key role in the maintainance of cellular Ca2+ homeostasis, alterations in the mitochondrial Ca2+ content ([Ca2+](m)) were monitored in addition to changes in cytosolic Ca2+ concentration ([Ca2+](i)) during metabolic inhibition (MI) in renal epithelial Madin-Darby canine kidney (MDCK) cells. [Ca2+](i) and [Ca2+](m) were monitored via, respectively, fura 2 and rhod 2 measurements. MI induced an increase in [Ca2+](i) reaching 631 +/- 78 nM in similar to20 min, followed by a decrease to 118 +/- 9 nM in the next similar to 25 min. A pronounced drop in cellular ATP levels and a rapid increase in intracellular Na+ concentrations in the first 20 min of MI excluded Ca2+ efflux in the second phase via plasma membrane ATPases or Na+/Ca2+ exchangers (NCE). Mitochondrial rhod 2 intensities increased to 434 +/- 46% of the control value during MI, indicating that mitochondria sequester Ca2+ during MI. The mitochondrial potential (DeltaPsi(m)) was lost in 20 min of MI, excluding mitochondrial Ca2+ uptake via the DeltaPsi(m)-dependent mitochondrial Ca2+ uniporter after 20 min of MI. Under Na+-free conditions, or when CGP-37157, a specific inhibitor of the mitochondrial NCE, was used, no drop in [Ca2+](i) was seen during MI, whereas the MI-induced increase in mitochondrial rhod 2 fluorescence was strongly reduced. To our knowledge, this study is the first to report that in metabolically inhibited renal epithelial cells mitochondria take up Ca2+ via the NCE acting in the reverse mode. | - |
dc.language.iso | en | - |
dc.publisher | AMER PHYSIOLOGICAL SOC | - |
dc.subject.other | renal epithelial cells; intramitochondrial calcium; rhod 2; CGP-37157 | - |
dc.title | Ca2+ uptake in mitochondria occurs via the reverse action of the Na+/Ca2+ exchanger in metabolically inhibited MDCK cells | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | F794 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | F784 | - |
dc.identifier.volume | 286 | - |
local.format.pages | 11 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Transnatl Univ Limburg, Limburgs Univ Ctr, Dept Physiol, Biomed Onderzoeksinst, B-3590 Diepenbeek, Belgium.Smets, I, Transnatl Univ Limburg, Limburgs Univ Ctr, Dept Physiol, Biomed Onderzoeksinst, Univ Campus Gebouw D, B-3590 Diepenbeek, Belgium.ilse.smets@luc.ac.be | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1152/ajprenal.00284.2003 | - |
dc.identifier.isi | 000220033100023 | - |
item.fulltext | No Fulltext | - |
item.contributor | SMETS, Ilse | - |
item.contributor | CAPLANUSI, Adrian | - |
item.contributor | Despa, S | - |
item.contributor | MOLNAR, Zsolt | - |
item.contributor | RADU, Mihai | - |
item.contributor | VAN DE VEN, Martin | - |
item.contributor | AMELOOT, Marcel | - |
item.contributor | STEELS, Paul | - |
item.accessRights | Closed Access | - |
item.validation | ecoom 2005 | - |
item.fullcitation | SMETS, Ilse; CAPLANUSI, Adrian; Despa, S; MOLNAR, Zsolt; RADU, Mihai; VAN DE VEN, Martin; AMELOOT, Marcel & STEELS, Paul (2004) Ca2+ uptake in mitochondria occurs via the reverse action of the Na+/Ca2+ exchanger in metabolically inhibited MDCK cells. In: AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 286(4). p. F784-F794. | - |
crisitem.journal.issn | 0363-6127 | - |
Appears in Collections: | Research publications |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.