The effect of physical training on maintenance and recovery of the functional muscular strength in rats injected with EAE

Abstract

The aim of this report was to study the effect of physical training on maintenance and recovery of the functional muscular strength in rats injected with Experimental Allergic Encephalomyelitis. For this effect 25 rats from different mothers and of the same gender were randomly selected. The 25 rats were again randomly assigned to two treatments groups. One group treated with EAE (13 rats) and the rest assigned to the control group (12 rats). Each treatment group was subdivided into two: swimmers and non swimmers. The data revealed a longitudinal set up that is repeated measures were performed on each rat over time (25 days) and to have an idea of the trend of the average behavior of the population of rats, a mean profile was plotted considering the time spent on the Rotorot as the main response. The profile revealed a non constant pattern of the evolution trend. Further, to determine the co-variance structure, a variance function was plotted and it also revealed a non constant pattern suggesting and unstructured type. Methodology: To capture the flexibility of the mean profile, a MACRO was implemented to generate fractional polynomial time variables. The obtained fractional time variables were used in PROC MIXED statement in SAS with the repeated option. Conclusion: We found that on an average point of view, Experimental Allergic Encephalomyelitis (EAE) has an effect that is; rats that were subjected to EAE had a different evolution pattern over time as compared to the ones in the control group. We also discovered that physical (training) has an effect on the functional strength of the rats. According to the results, rats that were subjected to EAE and who were undergoing physical training spent more time on the Rotorot than rats that were not subjected to physical training.