Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3767
Title: DEVELOPMENT AND FUNCTIONAL-CHARACTERIZATION OF A MURINE HUMAN CHIMERIC ANTIBODY WITH SPECIFICITY FOR THE HUMAN INTERLEUKIN-2 RECEPTOR
Authors: VANDEVYVER, Caroline
STEUKERS, M
LAMBRECHT, Johan 
HEYLIGEN, Harie 
RAUS, Jef 
Issue Date: 1993
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Source: MOLECULAR IMMUNOLOGY, 30(10). p. 865-876
Abstract: A murine/human chimeric antibody, with specificity for the human interleukin-2 receptor, was developed by genetic engineering. For this purpose, the light and heavy chain variable region exons encoding the murine monoclonal antibody 2C8 were isolated and inserted into expression vectors containing the human kappa and gamma-1 constant regions. After transfection by electroporation of the chimeric genes into murine Sp 2/0 hybridoma cells, transfectomas secreting the complete chimeric antibody were selected. The chimeric antibody has similar binding properties as the original murine antibody. The in vitro cytotoxic effects of the murine and the chimeric antibodies were compared.
Notes: LIMBURG UNIV CENTRUM,B-3590 DIEPENBEEK,BELGIUM.VANDEVYVER, C, DR L WILLEMS INST,UNIV CAMPUS,B-3590 DIEPENBEEK,BELGIUM.
Document URI: http://hdl.handle.net/1942/3767
ISI #: A1993LP87800002
Type: Journal Contribution
Appears in Collections:Non-affiliated authors

Show full item record

WEB OF SCIENCETM
Citations

7
checked on May 14, 2022

Page view(s)

50
checked on May 18, 2022

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.