TABLE 1 in Is ' everything everywhere'? Unprecedented cryptic diversity in the cosmopolitan flatworm Gyratrix hermaphroditus

Abstract

TABLE 1 Primers and PCR protocols PrimersSequence (5'→3')UsageReference18STimAAMCTGGTTGATCCTGCCAGPCR/SeqNorén and Jondelius (1999)TimBTGATCCATCTGCAGGTTCACCTPCR/SeqNorén and Jondelius (1999)TimA/TimB PCR regime:5 min 10 s at 95°C, 30x (30 s at 94°C, 30 s at 55°C, 90 s at 72°C), 5 min at 72°C600FGGTGCCAGCAGCCGCGGTSeqWillems et al. (2006)600RACCGCGGCTGCTGGCACCSeqWillems et al. (2006)1100FCAGAGGTTCGAAGACGATCSeqNorén and Jondelius (1999)1100RGATCGTCTTCGAACCTCTGSeqNorén and Jondelius (1999)18S7FGCAATAACAGGTCTGTGATGCSeqNorén and Jondelius (1999)18S7FKGCATCACAGACCTGTTATTGCSeqNorén and Jondelius (1999)TimBTGATCCATCTGCAGGTTCACCTSeqNorén and Jondelius (1999)28SLSU5TAGGTCGACCCGCTGAAYTTAPCR/SeqLittlewood et al. (2000)LSUD6-3GGAACCCTTCTCCACTTCAGTCPCR/SeqLittlewood et al. (2000)LSU5/LSUD6-3 PCR regime:5 min at 95°C, 30x (60 s at 94°C, 60 s at 50°C, 90 s at 72°C), 5 min at 72°CL300FCAAGTACCGTGAGGGAAAGTTGSeqLittlewood et al. (2000)L300RCAACTTTCCCTCACGGTACTTGSeqLittlewood et al. (2000)L1600FGCAGGACGGTGGCCATGGAAGSeqLittlewood et al. (2000)L1600RCTTCCATGGCCACCGTCCTGCSeqLittlewood et al. (2000)5.8S+ITS258SITS2F1GCGGTGGATCACTCGGCTCGPCR/SeqThis study58SITS2R1TCGCTCGCCGCTACTRRGGGAPCR/SeqThis study58ITS2F/58ITS2R PCR regime:5 min at 95°C, 35x (60 s at 94°C, 60 s at 55°C, 72 s at 72°C), 5 min at 72°C