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http://hdl.handle.net/1942/1000
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DC Field | Value | Language |
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dc.contributor.author | MANGIN, Jean-Marie | - |
dc.contributor.author | Nguyen, L. | - |
dc.contributor.author | Gougnard, C. | - |
dc.contributor.author | Rogister, B. | - |
dc.contributor.author | Belachew, S. | - |
dc.contributor.author | Moonen, G. | - |
dc.contributor.author | RIGO, Jean-Michel | - |
dc.date.accessioned | 2006-11-19T21:45:07Z | - |
dc.date.available | 2006-11-19T21:45:07Z | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | MOLECULAR PHARMACOLOGY, 67(5). p. 1783-1796 | - |
dc.identifier.issn | 0026-895X | - |
dc.identifier.uri | http://hdl.handle.net/1942/1000 | - |
dc.description.abstract | In this work, we show that beta-carbolines, which are known negative allosteric modulators of GABA A receptors, inhibit glycine-induced currents of embryonic mouse spinal cord and hippocampal neurons. In both cell types, beta-carboline-induced inhibition of glycine receptor (GlyR)-mediated responses decreases with time in culture. Single-channel recordings show that the major conductance levels of GlyR unitary currents shifts from high levels (>= 50 pS) in 2 to 3 days in vitro (DIV) neurons to low levels (< 50 pS) in 11 to 14 DIV neurons, assessing the replacement of functional homomeric GlyR by heteromeric GlyR. In cultured spinal cord neurons, the disappearance of beta-carboline inhibition of glycine responses and high conductance levels is almost complete in mature neurons, whereas a weaker decrease in beta-carboline-evoked glycine response inhibition and high conductance level proportion is observed in hippocampal neurons. To confirm the hypothesis that the decreased sensitivity of GlyR to beta-carbolines depends on beta subunit expression, Chinese hamster ovary cells were permanently transfected either with GlyR alpha 2 subunit alone or in combination with GlyR beta subunit. Single-channel recordings revealed that the major conductance levels shifted from high levels (>= 50 pS) in GlyR-alpha 2-transfected cells to low levels (< 50 pS) in GlyR-alpha 2-containing cells. Consistently, both picrotoxinand beta-carboline-induced inhibition of glycine-gated currents were significantly decreased in GlyR-alpha 2-transfected cells compared with GlyR-alpha 2-containing cells. In summary, we demonstrate that the incorporation of beta subunits in GlyRs confers resistance not only to picrotoxin but also to beta-carbolineinduced inhibition. Furthermore, we also provide evidence that hippocampal neurons undergo in vitro a partial maturation process of their GlyR-mediated responses. | - |
dc.format.extent | 2311084 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | Bethesda : AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS | - |
dc.subject.other | RAT SPINAL-CORD; BENZODIAZEPINE RECEPTORS; SYNAPTIC-TRANSMISSION; IN-VITRO; NEURONS; CURRENTS; MODULATION; LIGAND; ADULT; BRAIN | - |
dc.title | Developmental regulation of beta-carboline-induced inhibition of glycine-evoked responses depends on glycine receptor beta subunit expression | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 1796 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 1783 | - |
dc.identifier.volume | 67 | - |
local.bibliographicCitation.jcat | A1 | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1124/mol.104.007435 | - |
dc.identifier.isi | 000228387900048 | - |
item.fullcitation | MANGIN, Jean-Marie; Nguyen, L.; Gougnard, C.; Rogister, B.; Belachew, S.; Moonen, G. & RIGO, Jean-Michel (2005) Developmental regulation of beta-carboline-induced inhibition of glycine-evoked responses depends on glycine receptor beta subunit expression. In: MOLECULAR PHARMACOLOGY, 67(5). p. 1783-1796. | - |
item.validation | ecoom 2006 | - |
item.contributor | MANGIN, Jean-Marie | - |
item.contributor | Nguyen, L. | - |
item.contributor | Gougnard, C. | - |
item.contributor | Rogister, B. | - |
item.contributor | Belachew, S. | - |
item.contributor | Moonen, G. | - |
item.contributor | RIGO, Jean-Michel | - |
item.fulltext | With Fulltext | - |
item.accessRights | Open Access | - |
crisitem.journal.issn | 0026-895X | - |
crisitem.journal.eissn | 1521-0111 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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developmental.pdf | Peer-reviewed author version | 2.26 MB | Adobe PDF | View/Open |
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