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http://hdl.handle.net/1942/1002
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DC Field | Value | Language |
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dc.contributor.author | SOMERS, Veerle | - |
dc.contributor.author | GOVARTS, Cindy | - |
dc.contributor.author | HELLINGS, Niels | - |
dc.contributor.author | Hupperts, R. | - |
dc.contributor.author | STINISSEN, Piet | - |
dc.date.accessioned | 2006-11-23T08:25:20Z | - |
dc.date.available | 2006-11-23T08:25:20Z | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | JOURNAL OF AUTOIMMUNITY, 25(3). p. 223-228 | - |
dc.identifier.issn | 0896-8411 | - |
dc.identifier.uri | http://hdl.handle.net/1942/1002 | - |
dc.description.abstract | The identification of disease related autoantigens targeted by pathogenic T- and B-cell responses is crucial for the development of improved therapies for autoimmune diseases. To identify immunogenic targets recognized by the humoral immune response, we have recently applied a novel and powerful molecular approach, named ‘serological antigen selection’ (SAS). This method involves the display of a cDNA expression library on filamentous phage and subsequent selection on patient immunoglobulin G (IgG). In the present study, we have cloned a cDNA repertoire from a multiple sclerosis (MS) patient in pVI phage display vectors and performed selections on pooled MS cerebrospinal fluid (CSF) samples immobilized with anti-human IgG. To further streamline this procedure, we report an optimized SAS procedure in which we have successfully established methods for enrichment of MS-specific candidate antigens. In conclusion, the broad applicability of the SAS method makes it a highly promising method for investigating the autoimmune repertoire. | - |
dc.format.extent | 317243 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | Elsevier | - |
dc.subject.other | Serological antigen selection; Filamentous phage; cDNA display; Multiple sclerosis; Autoantibody repertoire; Autoimmune diseases | - |
dc.title | Profiling the autoantibody repertoire by serological antigen selection | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 228 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 223 | - |
dc.identifier.volume | 25 | - |
local.bibliographicCitation.jcat | A1 | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1016/j.jaut.2005.09.023 | - |
dc.identifier.isi | 000233891200008 | - |
item.validation | ecoom 2006 | - |
item.accessRights | Open Access | - |
item.fullcitation | SOMERS, Veerle; GOVARTS, Cindy; HELLINGS, Niels; Hupperts, R. & STINISSEN, Piet (2005) Profiling the autoantibody repertoire by serological antigen selection. In: JOURNAL OF AUTOIMMUNITY, 25(3). p. 223-228. | - |
item.fulltext | With Fulltext | - |
item.contributor | SOMERS, Veerle | - |
item.contributor | GOVARTS, Cindy | - |
item.contributor | HELLINGS, Niels | - |
item.contributor | Hupperts, R. | - |
item.contributor | STINISSEN, Piet | - |
crisitem.journal.issn | 0896-8411 | - |
crisitem.journal.eissn | 1095-9157 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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Profiling.pdf | Peer-reviewed author version | 309.81 kB | Adobe PDF | View/Open |
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