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Title: | Biological modification of carbon nanowalls with DNA strands and hybridization experiments with complementary and mismatched DNA | Authors: | VANSWEEVELT, Rob Malesevic, A. VAN GOMPEL, Matthias Vanhulsel, A. WENMACKERS, Sylvia D'HAEN, Jan VERMEEREN, Veronique AMELOOT, Marcel MICHIELS, Luc Van Haesendonck, C. WAGNER, Patrick |
Issue Date: | 2010 | Publisher: | ELSEVIER SCIENCE BV | Source: | CHEMICAL PHYSICS LETTERS, 485(1-3). p. 196-201 | Abstract: | Carbon nanowalls (CNWs) are functionalized with short DNA strands. CNWs are a network of upstanding carbon flakes consisting of 4-6 graphene layers packed together. The biological modi. cation of these structures is carried out via the photochemical attachment of an unsaturated fatty acid linker molecule, followed by an EDC-mediated reaction to bind amino-modified DNA strands to the linker. Hybridization experiments are done using complementary and mismatch DNA strands. Fluorescence microscopy shows a clear difference between EDC-negative and EDC-positive samples, supporting the covalent nature of the DNA attachment. Furthermore, we demonstrate that the hybridization is highly selective and reproducible. (C) 2009 Elsevier B. V. All rights reserved. | Notes: | [Vansweevelt, R.; Van Gompel, M.; Wenmackers, S.; D'Haen, J.; Wagner, P.] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium. [Malesevic, A.; Vanhulsel, A.] Flemish Inst Technol Res, VITO Mat, B-2400 Mol, Belgium. [Malesevic, A.; Van Haesendonck, C.] Katholieke Univ Leuven, Lab Solid State Phys & Magnetism, B-3001 Louvain, Belgium. [Vermeeren, V.; Ameloot, M.; Michiels, L.] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [Wagner, P.] IMEC, Div IMOMEC, B-3590 Diepenbeek, Belgium. rob.vansweevelt@uhasselt.be | Document URI: | http://hdl.handle.net/1942/10434 | ISSN: | 0009-2614 | e-ISSN: | 1873-4448 | DOI: | 10.1016/j.cplett.2009.12.040 | ISI #: | 000273231900040 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2011 |
Appears in Collections: | Research publications |
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