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|Title:||Low Current-driven Micro-electroporation Allows Efficient In Vivo Delivery of Nonviral DNA into the Adult Mouse Brain||Authors:||DE VRY, Jochen
Bode, Gerard H.
Steinbusch, Harry W. M.
DE BAETS, Marc
|Issue Date:||2010||Publisher:||NATURE PUBLISHING GROUP||Source:||MOLECULAR THERAPY, 18 (6). p. 1183-1191||Abstract:||Viral gene transfer or transgenic animals are commonly used technologies to alter gene expression in the adult brain, although these approaches lack spatial specificity and are time consuming. We delivered plasmid DNA locally into the brain of adult C57BL/6 mice in vivo by voltage-and current-controlled electroporation. The low current-controlled delivery of unipolar square wave pulses of 125 mu A with microstimulation electrodes at the injection site gave 16 times higher transfection rates than a voltage-controlled electroporation protocol with plate electrodes resulting in currents of about 400 mA. Transfection was restricted to the target region and no damage due to the electric pulses was found. Our current-controlled electroporation protocol indicated that the use of very low currents resulting in applied voltages within the physiological range of the membrane potential, allows efficient transfection of nonviral plasmid DNA. In conclusion, low current-controlled electroporation is an excellent approach for electroporation in the adult brain, i.e., gene function can be influenced locally at a high level with no mortality and minimal tissue damage.||Notes:||[De Vry, Jochen; Martinez-Martinez, Pilar; Losen, Mario; Bode, Gerard H.; Temel, Yasin; Steinbusch, Harry W. M.; De Baets, Marc; Prickaerts, Jos] Maastricht Univ, Dept Psychiat & Neuropsychol, Sch Mental Hlth & Neurosci, NL-6200 MD Maastricht, Netherlands. [De Vry, Jochen; Martinez-Martinez, Pilar; Losen, Mario; Bode, Gerard H.; Temel, Yasin; Steinbusch, Harry W. M.; De Baets, Marc; Prickaerts, Jos] Maastricht Univ, European Grad Sch Neurosci EURON, NL-6200 MD Maastricht, Netherlands. [Temel, Yasin] Maastricht Univ, Med Ctr, Dept Neurosurg, NL-6200 MD Maastricht, Netherlands. [Steckler, Thomas] Johnson & Johnson PRD, Dept Psychiat, Beerse, Belgium. [De Baets, Marc] Hasselt Univ, Sch Life Sci, Biomed Res Inst, Hasselt, Belgium. [De Baets, Marc] Hasselt Univ, Translat Univ Limburg, Hasselt, Belgium. firstname.lastname@example.org||Document URI:||http://hdl.handle.net/1942/10981||ISSN:||1525-0016||e-ISSN:||1525-0024||DOI:||10.1038/mt.2010.62||ISI #:||000278545800016||Category:||A1||Type:||Journal Contribution||Validations:||ecoom 2011|
|Appears in Collections:||Research publications|
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