Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/11025
Full metadata record
DC FieldValueLanguage
dc.contributor.authorVan Dorst, Bieke-
dc.contributor.authorMehta, Jaytry-
dc.contributor.authorRouah-Martin, Elsa-
dc.contributor.authorSOMERS, Veerle-
dc.contributor.authorDe Coen, Wim-
dc.contributor.authorBlust, Ronny-
dc.contributor.authorRobbens, Johan-
dc.date.accessioned2010-08-03T07:33:52Z-
dc.date.availableNO_RESTRICTION-
dc.date.available2010-08-03T07:33:52Z-
dc.date.issued2010-
dc.identifier.citationTOXICOLOGY IN VITRO, 24(5). p. 1435-1440-
dc.identifier.issn0887-2333-
dc.identifier.urihttp://hdl.handle.net/1942/11025-
dc.description.abstractcDNA phage display is frequently used in drug development to screen for cellular target of drugs. However, in toxicology, cDNA phage display remains unexplored, although it has large potential in this field. In this study, cDNA phage display is demonstrated as a novel tool to screen for interactions between chemical compounds and cellular targets. The knowledge of these target interactions is valuable to have a more complete understanding of the mechanisms of action of chemical compounds. Bisphenol A (BPA) was selected as a model compound for this study. By selection of the cellular proteins that bind BPA with cDNA phage display, it was possible to identify a known cellular target of BPA, tubulin a and a possible novel cellular target of BPA, transforming acidic coiled-coil containing protein 3. Both these cellular proteins are involved in the mechanism of cell division. The disruption of cell division is a known non-genomic effect of BPA. Non-genomic effects are not mediated by differences in gene expression and therefore important mechanistic information might be missed with the widely used differential gene expression techniques for mode of action research. This cDNA phage display technique can provide important additional information about the interaction of chemical compounds with cellular targets that mediates these non-genomic actions and therefore gives complementary information to toxicogenomic studies to obtain a more complete understanding of the mechanism of action of chemical compounds. (C) 2010 Elsevier Ltd. All rights reserved.-
dc.description.sponsorshipThe present study was financially supported by the 'Institute for the promotion of innovation by science and technology (IWT)' in Flanders (Belgium) and by the Federal Public Service of Health, Food Chain Safety and Environment (contract RT 07/11 INVITRAB and RF6204 ERGOT). The authors like to thank Prof. Jeffrey B. Arterburn (New Mexico State University) for providing the biotinylated BPA.-
dc.language.isoen-
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD-
dc.subject.othercDNA phage display; Cellular targets; Bisphenol A; Non-genomic action; Chemical compounds-
dc.subject.othercDNA phage display; Cellular targets; Bisphenol A; Non-genomic action; Chemical compounds-
dc.titlecDNA phage display as a novel tool to screen for cellular targets of chemical compounds-
dc.typeJournal Contribution-
dc.identifier.epage1440-
dc.identifier.issue5-
dc.identifier.spage1435-
dc.identifier.volume24-
local.format.pages6-
local.bibliographicCitation.jcatA1-
dc.description.notes[Van Dorst, Bieke; Mehta, Jaytry; Rouah-Martin, Elsa; De Coen, Wim; Blust, Ronny; Robbens, Johan] Univ Antwerp, Dept Biol, Lab Ecophysiol Biochem & Toxicol, B-2020 Antwerp, Belgium. [Van Dorst, Bieke; Mehta, Jaytry; Rouah-Martin, Elsa; Robbens, Johan] Inst Agr & Fisheries Res ILVO, B-8400 Oostende, Belgium. [Somers, Veerle] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [De Coen, Wim] European Chem Agcy ECHA, F-00120 Helsinki, Finland. Bieke.vandorst@ua.ac.be-
local.type.refereedRefereed-
local.type.specifiedArticle-
dc.bibliographicCitation.oldjcatA1-
dc.identifier.doi10.1016/j.tiv.2010.04.003-
dc.identifier.isi000279650600014-
item.fulltextWith Fulltext-
item.fullcitationVan Dorst, Bieke; Mehta, Jaytry; Rouah-Martin, Elsa; SOMERS, Veerle; De Coen, Wim; Blust, Ronny & Robbens, Johan (2010) cDNA phage display as a novel tool to screen for cellular targets of chemical compounds. In: TOXICOLOGY IN VITRO, 24(5). p. 1435-1440.-
item.contributorVan Dorst, Bieke-
item.contributorMehta, Jaytry-
item.contributorRouah-Martin, Elsa-
item.contributorSOMERS, Veerle-
item.contributorDe Coen, Wim-
item.contributorBlust, Ronny-
item.contributorRobbens, Johan-
item.validationecoom 2011-
item.accessRightsRestricted Access-
crisitem.journal.issn0887-2333-
crisitem.journal.eissn1879-3177-
Appears in Collections:Research publications
Files in This Item:
File Description SizeFormat 
somers 1.pdf
  Restricted Access
Published version312.74 kBAdobe PDFView/Open    Request a copy
Show simple item record

SCOPUSTM   
Citations

20
checked on Sep 7, 2020

WEB OF SCIENCETM
Citations

21
checked on Mar 21, 2024

Page view(s)

132
checked on Sep 7, 2022

Download(s)

120
checked on Sep 7, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.