Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/11132
Title: Molecular and physiological analysis of three Pseudomonas aeruginosa phages belonging to the "N4-like viruses"
Authors: Ceyssens, Pieter-Jan
Brabban, Andrew
Rogge, Larissa
Lewis, Matthew Spooner
Pickard, Derek
Goulding, David
Dougan, Gordon
NOBEN, Jean-Paul 
Kropinski, Andrew
Kutter, Elizabeth
Lavigne, Rob
Issue Date: 2010
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Source: VIROLOGY, 405 (1). p. 26-30
Abstract: We present a detailed analysis of the genome architecture, structural proteome and infection-related properties of three Pseudomonas phages, designated LUZ7, LIT1 and PEV2 These podoviruses encapsulate 72 5 to 749 kb genomes and lyse their host after 25 min aerobic infection PEV2 can successfully infect under anaerobic conditions, but its latent period is tripled, the lysis proceeds far slower and the burst size decreases significantly While the overall genome structure of these phages resembles the well-studied coliphage N4, these Pseudomonas phages encode a cluster of tail genes which displays significant similarity to a Pseudomonas aeruginosa (cryptic) prophage region Using ESI-MS/MS, these tail proteins were shown to be part of the phage particle, as well as ten other proteins including a giant 370 kDa virion RNA polymerase These phages are the first described representatives of a novel kind of obligatory lytic P aeruginosa-infecting phages, belonging to the widespread "N4-like viruses" genus (C) 2010 Elsevier Inc All rights reserved
Notes: Lavigne, R (reprint author), Katholieke Univ Leuven, Div Gene Technol, Kasteelpk Arenberg 21, B-3001 Louvain, Belgium. [Ceyssens, Pieter-Jan; Lavigne, Rob] Katholieke Univ Leuven, Div Gene Technol, B-3001 Louvain, Belgium. [Brabban, Andrew; Rogge, Larissa; Lewis, Matthew Spooner; Kutter, Elizabeth] Evergreen State Coll, Phage Biol Lab, Olympia, WA 98505 USA. [Pickard, Derek; Goulding, David; Dougan, Gordon] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England. [Noben, Jean-Paul] Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium. [Noben, Jean-Paul] Transnat Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. [Kropinski, Andrew] Lab Foodborne Zoonoses, Guelph, ON N1G 3W4, Canada.
Keywords: Bacteriophage; Genomics; Anaerobic infection; Pseudomonas aeruginosa; Phage N4; Mass spectrometry;Bacteriophage; Genomics; Anaerobic infection; Pseudomonas aeruginosa; Phage N4; Mass spectrometry
Document URI: http://hdl.handle.net/1942/11132
ISSN: 0042-6822
e-ISSN: 1089-862X
DOI: 10.1016/j.virol.2010.06.011
ISI #: 000280691700005
Category: A1
Type: Journal Contribution
Validations: ecoom 2011
Appears in Collections:Research publications

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