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Title: | Molecular and physiological analysis of three Pseudomonas aeruginosa phages belonging to the "N4-like viruses" | Authors: | Ceyssens, Pieter-Jan Brabban, Andrew Rogge, Larissa Lewis, Matthew Spooner Pickard, Derek Goulding, David Dougan, Gordon NOBEN, Jean-Paul Kropinski, Andrew Kutter, Elizabeth Lavigne, Rob |
Issue Date: | 2010 | Publisher: | ACADEMIC PRESS INC ELSEVIER SCIENCE | Source: | VIROLOGY, 405 (1). p. 26-30 | Abstract: | We present a detailed analysis of the genome architecture, structural proteome and infection-related properties of three Pseudomonas phages, designated LUZ7, LIT1 and PEV2 These podoviruses encapsulate 72 5 to 749 kb genomes and lyse their host after 25 min aerobic infection PEV2 can successfully infect under anaerobic conditions, but its latent period is tripled, the lysis proceeds far slower and the burst size decreases significantly While the overall genome structure of these phages resembles the well-studied coliphage N4, these Pseudomonas phages encode a cluster of tail genes which displays significant similarity to a Pseudomonas aeruginosa (cryptic) prophage region Using ESI-MS/MS, these tail proteins were shown to be part of the phage particle, as well as ten other proteins including a giant 370 kDa virion RNA polymerase These phages are the first described representatives of a novel kind of obligatory lytic P aeruginosa-infecting phages, belonging to the widespread "N4-like viruses" genus (C) 2010 Elsevier Inc All rights reserved | Notes: | Lavigne, R (reprint author), Katholieke Univ Leuven, Div Gene Technol, Kasteelpk Arenberg 21, B-3001 Louvain, Belgium. [Ceyssens, Pieter-Jan; Lavigne, Rob] Katholieke Univ Leuven, Div Gene Technol, B-3001 Louvain, Belgium. [Brabban, Andrew; Rogge, Larissa; Lewis, Matthew Spooner; Kutter, Elizabeth] Evergreen State Coll, Phage Biol Lab, Olympia, WA 98505 USA. [Pickard, Derek; Goulding, David; Dougan, Gordon] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England. [Noben, Jean-Paul] Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium. [Noben, Jean-Paul] Transnat Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. [Kropinski, Andrew] Lab Foodborne Zoonoses, Guelph, ON N1G 3W4, Canada. | Keywords: | Bacteriophage; Genomics; Anaerobic infection; Pseudomonas aeruginosa; Phage N4; Mass spectrometry;Bacteriophage; Genomics; Anaerobic infection; Pseudomonas aeruginosa; Phage N4; Mass spectrometry | Document URI: | http://hdl.handle.net/1942/11132 | ISSN: | 0042-6822 | e-ISSN: | 1089-862X | DOI: | 10.1016/j.virol.2010.06.011 | ISI #: | 000280691700005 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2011 |
Appears in Collections: | Research publications |
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