Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/11306
Title: | A novel method for making human monoclonal antibodies | Authors: | FRAUSSEN, Judith Vrolix, K. Martinez-Martinez, Pilar Losen, M. Meulemans, E. DE BAETS, Marc STINISSEN, Piet SOMERS, Veerle |
Issue Date: | 2010 | Publisher: | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Source: | JOURNAL OF AUTOIMMUNITY, 35 (2). p. 130-134 | Abstract: | We have developed a B cell immortalization method for low B cell numbers per well using simultaneous B cell stimulation by CpG2006 and B cell infection by Epstein-Barr virus (EBV), followed by an additional CpG2006 and interleukin-2 (IL-2) stimulus. Using this method, immunoglobulin G (IgG)-producing immortalized B cell lines were generated from peripheral blood IgG(+)CD22(+) B cells with an efficiency of up to 83%. Antibody can already be obtained from the culture supernatant after 3-4 weeks. Moreover, clonality analysis demonstrated monoclonality in 87% of the resulting immortalized B cell lines. Given the high immortalization efficiency and monoclonality rate, evidence is provided that no further sub-cloning is necessary. An important application of this B cell immortalization method is the characterization of (autoreactive) antibodies from patients with autoimmune disease. This could eventually lead to the identification of new autoantigens, disease markers or targets for therapy. (C) 2010 Elsevier Ltd. All rights reserved. | Notes: | [Fraussen, J.; De Baets, M. H.; Stinissen, P.; Somers, V.] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [Fraussen, J.; De Baets, M. H.; Stinissen, P.; Somers, V.] Transnatl Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. [Vrolix, K.; Martinez-Martinez, P.; Losen, M.; De Baets, M. H.] Maastricht Univ, Sch Mental Hlth & Neurosci, Dept Neurosci, Maastricht, Netherlands. [Meulemans, E.] Univ Limburg, Acad Hosp Maastricht, Dept Pathol, Maastricht, Netherlands. veerle.somers@uhasselt.be | Keywords: | B cell immortalization; Epstein-Barr virus; Monoclonal antibodies; B cell spectratyping;B cell immortalization; Epstein-Barr virus; Monoclonal antibodies; B cell spectratyping | Document URI: | http://hdl.handle.net/1942/11306 | ISSN: | 0896-8411 | e-ISSN: | 1095-9157 | DOI: | 10.1016/j.jaut.2010.05.001 | ISI #: | 000281986100004 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2011 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
de baets 1.pdf Restricted Access | Published version | 292.18 kB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
17
checked on Sep 2, 2020
WEB OF SCIENCETM
Citations
14
checked on Mar 21, 2024
Page view(s)
74
checked on Aug 31, 2022
Download(s)
48
checked on Aug 31, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.