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http://hdl.handle.net/1942/11473
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DC Field | Value | Language |
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dc.contributor.author | HAENEN, Steven | - |
dc.contributor.author | Vanoirbeek, Jeroen A. J. | - |
dc.contributor.author | De Vooght, Vanessa | - |
dc.contributor.author | Maes, Evelyne | - |
dc.contributor.author | Schools, Liliane | - |
dc.contributor.author | Nemery, Benoit | - |
dc.contributor.author | Hoet, Peter H. M. | - |
dc.contributor.author | CLYNEN, Elke | - |
dc.date.accessioned | 2011-01-09T08:45:21Z | - |
dc.date.available | NO_RESTRICTION | - |
dc.date.available | 2011-01-09T08:45:21Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | JOURNAL OF PROTEOME RESEARCH, 9 (11). p. 5868-5876 | - |
dc.identifier.issn | 1535-3893 | - |
dc.identifier.uri | http://hdl.handle.net/1942/11473 | - |
dc.description.abstract | Occupational asthma is the principal cause of work-related respiratory disease in the industrial world. Toluene-2,4-diisocyanate (TDI) is one of the most common respiratory sensitizers leading to occupational asthma. Using a mouse model of chemical-induced asthma, we explored proteome changes in multiple compartments of mice sensitized and challenged with TDI or acetone-olive oil (AOO; vehicle). Airway reactivity to methacholine and a bronchoalveolar lavage (BAL) cell count was assessed in treated and control mice, 1 day after challenge. Subsequently, two-dimensional differential gel electrophoresis (2D-DIGE) was performed on auricular lymph nodes, BAL, and serum comparing TDI-treated and vehicletreated control mice. The differentially expressed proteins were identified by mass spectrometry and pathway analysis was performed. TDI-treated mice exhibit increased airway reactivity (2.6-fold increase) and a neutrophilic inflammation in the BAL fluid, compared to control mice. 2D-DIGE showed 53, 210, and 40 differentially expressed proteins in the auricular lymph nodes, BAL, and serum of TDI-treated versus vehicle-treated mice, respectively. Several of the identified proteins could be linked with inflammation, neutrophil chemotaxis, and/or oxidative stress. Physiologic and immunologic readouts of the asthmatic phenotype, such as inflammation, were confirmed in three compartments by several of the differentially expressed proteins via 2D-DIGE and computerized pathway analysis. | - |
dc.description.sponsorship | Special thanks to Koen Vandingenen for the technical assistance with the 2D-DIGE analysis. The project was supported by a grant of the Interuniversity Attraction Pole Program, Belgian State, Belgian Science Policy [P6/35] and from the 'Fund for Scientific Research Flanders' (FWO), [FWO G.0547.08]. J.A.J.V. is a postdoctoral fellow of the FWD. | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject.other | 2D-DIGE; auricular lymph nodes; BALB/c mouse; bronchoalveolar lavage; serum; toluene-2,4-diisocyanate | - |
dc.subject.other | 2D-DIGE; auricular lymph nodes; BALB/c mouse; bronchoalveolar lavage; serum; toluene-2,4-diisocyanate | - |
dc.title | Proteome Analysis of Multiple Compartments in a Mouse Model of Chemical-Induced Asthma | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 5876 | - |
dc.identifier.issue | 11 | - |
dc.identifier.spage | 5868 | - |
dc.identifier.volume | 9 | - |
local.format.pages | 9 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Haenen, Steven; Vanoirbeek, Jeroen A. J.; De Vooght, Vanessa; Nemery, Benoit; Hoet, Peter H. M.] Katholieke Univ Leuven, Res Unit Lung Toxicol, B-3000 Louvain, Belgium. [Haenen, Steven; Maes, Evelyne; Schools, Liliane] Katholieke Univ Leuven, Res Grp Funct Genom & Prote, B-3000 Louvain, Belgium. [Clynen, Elke] Hasselt Univ, BIOMED Res Inst, B-3590 Diepenbeek, Belgium. peter.hoet@med.kuleuven.be | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1021/pr100638m | - |
dc.identifier.isi | 000283810500035 | - |
item.validation | ecoom 2011 | - |
item.fulltext | No Fulltext | - |
item.accessRights | Closed Access | - |
item.fullcitation | HAENEN, Steven; Vanoirbeek, Jeroen A. J.; De Vooght, Vanessa; Maes, Evelyne; Schools, Liliane; Nemery, Benoit; Hoet, Peter H. M. & CLYNEN, Elke (2010) Proteome Analysis of Multiple Compartments in a Mouse Model of Chemical-Induced Asthma. In: JOURNAL OF PROTEOME RESEARCH, 9 (11). p. 5868-5876. | - |
item.contributor | HAENEN, Steven | - |
item.contributor | Vanoirbeek, Jeroen A. J. | - |
item.contributor | De Vooght, Vanessa | - |
item.contributor | Maes, Evelyne | - |
item.contributor | Schools, Liliane | - |
item.contributor | Nemery, Benoit | - |
item.contributor | Hoet, Peter H. M. | - |
item.contributor | CLYNEN, Elke | - |
crisitem.journal.issn | 1535-3893 | - |
crisitem.journal.eissn | 1535-3907 | - |
Appears in Collections: | Research publications |
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