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http://hdl.handle.net/1942/11853
Title: | Transcriptome analysis by cDNA-AFLP of Suillus luteus Cd-tolerant and Cd-sensitive isolates | Authors: | RUYTINX, Joske Craciun, Adrian R. VERSTRAELEN, Karen VANGRONSVELD, Jaco COLPAERT, Jan Verbruggen, Nathalie |
Issue Date: | 2011 | Publisher: | SPRINGER | Source: | MYCORRHIZA, 21(3). p. 145-154 | Abstract: | The ectomycorrhizal basidiomycete Suillus luteus (L.:Fr.), a typical pioneer species which associates with young pine trees colonizing disturbed sites, is a common root symbiont found at heavy metal contaminated sites. Three Cd-sensitive and three Cd-tolerant isolates of S. luteus, isolated respectively from non-polluted and a heavy metal-polluted site in Limburg (Belgium), were used for a transcriptomic analysis. We identified differentially expressed genes by cDNA-AFLP analysis. The possible roles of some of the encoded proteins in heavy metal (Cd) accumulation and tolerance are discussed. Despite the high conservation of coding sequences in S. luteus, a large intraspecific variation in the transcript profiles was observed. This variation was as large in Cd-tolerant as in sensitive isolates and may help this pioneer species to adapt to novel environments. | Notes: | [Craciun, Adrian R.; Verbruggen, Nathalie] Univ Libre Bruxelles, Lab Physiol & Genet Mol Plantes, B-1050 Brussels, Belgium. [Ruytinx, Joske; Verstraelen, Karen; Vangronsveld, Jaco; Colpaert, Jan V.] Univ Hasselt, Environm Biol Grp, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. | Keywords: | Cd-tolerance; Suillus luteus; cDNA-AFLP; Transcript profiling | Document URI: | http://hdl.handle.net/1942/11853 | ISSN: | 0940-6360 | e-ISSN: | 1432-1890 | DOI: | 10.1007/s00572-010-0318-2 | ISI #: | 000288459400001 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2012 |
Appears in Collections: | Research publications |
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