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|Title:||Transcriptome analysis by cDNA-AFLP of Suillus luteus Cd-tolerant and Cd-sensitive isolates||Authors:||RUYTINX, Joske
Craciun, Adrian R.
|Issue Date:||2011||Publisher:||SPRINGER||Source:||MYCORRHIZA, 21(3). p. 145-154||Abstract:||The ectomycorrhizal basidiomycete Suillus luteus (L.:Fr.), a typical pioneer species which associates with young pine trees colonizing disturbed sites, is a common root symbiont found at heavy metal contaminated sites. Three Cd-sensitive and three Cd-tolerant isolates of S. luteus, isolated respectively from non-polluted and a heavy metal-polluted site in Limburg (Belgium), were used for a transcriptomic analysis. We identified differentially expressed genes by cDNA-AFLP analysis. The possible roles of some of the encoded proteins in heavy metal (Cd) accumulation and tolerance are discussed. Despite the high conservation of coding sequences in S. luteus, a large intraspecific variation in the transcript profiles was observed. This variation was as large in Cd-tolerant as in sensitive isolates and may help this pioneer species to adapt to novel environments.||Notes:||[Craciun, Adrian R.; Verbruggen, Nathalie] Univ Libre Bruxelles, Lab Physiol & Genet Mol Plantes, B-1050 Brussels, Belgium. [Ruytinx, Joske; Verstraelen, Karen; Vangronsveld, Jaco; Colpaert, Jan V.] Univ Hasselt, Environm Biol Grp, Ctr Environm Sci, B-3590 Diepenbeek, Belgium.||Keywords:||Cd-tolerance; Suillus luteus; cDNA-AFLP; Transcript profiling||Document URI:||http://hdl.handle.net/1942/11853||ISSN:||0940-6360||e-ISSN:||1432-1890||DOI:||10.1007/s00572-010-0318-2||ISI #:||000288459400001||Category:||A1||Type:||Journal Contribution||Validations:||ecoom 2012|
|Appears in Collections:||Research publications|
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