Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/12066
Title: Unraveling uranium induced oxidative stress related responses in Arabidopsis thaliana seedlings. Part I: responses in the roots
Authors: VANHOUDT, Nathalie 
Vandenhove, Hildegarde
HOREMANS, Nele 
REMANS, Tony 
OPDENAKKER, Kelly 
SMEETS, Karen 
Bello, Daniel Martinez
Wannijn, Jean
Van Hees, May
VANGRONSVELD, Jaco 
CUYPERS, Ann 
Issue Date: 2011
Publisher: ELSEVIER SCI LTD
Source: JOURNAL OF ENVIRONMENTAL RADIOACTIVITY, 102(6). p. 630-637
Abstract: When aiming to evaluate the environmental impact of uranium contamination, it is important to unravel the mechanisms by which plants respond to uranium stress. As oxidative stress seems an important modulator under other heavy metal stress, this study aimed to investigate oxidative stress related responses in Arabidopsis thaliana exposed to uranium concentrations ranging from 0.1 to 100 mu M for 1, 3 and 7 days. Besides analyzing relevant reactive oxygen species-producing and -scavenging enzymes at protein and transcriptional level, the importance of the ascorbate-glutathione cycle under uranium stress was investigated. These results are reported separately for roots and leaves in two papers: Part I dealing with responses in the roots and Part II unraveling responses in the leaves and presenting general conclusions. Results of Part I indicate that oxidative stress related responses in the roots were only triggered following exposure to the highest uranium concentration of 100 mu M. A fast oxidative burst was suggested based on the observed enhancement of lipoxygenase (LOX1) and respiratory burst oxydase homolog (RBOHD) transcript levels already after 1 day. The first line of defense was attributed to superoxide dismutase (SOD), also triggered from the first day. The enhanced SOD-capacity observed at protein level corresponded with an enhanced expression of iron SOD (FSD1) located in the plastids. For the detoxification of H(2)O(2), an early increase in catalase (CAT1) transcript levels was observed while peroxidase capacities were enhanced at the later stage of 3 days. Although the ascorbate peroxidase capacity and gene expression (APX1) increased, the ascorbate/dehydroascorbate redox balance was completely disrupted and shifted toward the oxidized form. This disrupted balance could not be inverted by the glutathione part of the cycle although the glutathione redox balance could be maintained. (C) 2011 Elsevier Ltd. All rights reserved.
Notes: [Vanhoudt, N; Vandenhove, H; Horemans, N; Wannijn, J; Van Hees, M] Belgian Nucl Res Ctr SCK CEN, B-2400 Mol, Belgium [Vanhoudt, N; Remans, T; Opdenakker, K; Smeets, K; Vangronsveld, J; Cuypers, A] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium [Bello, DM] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, B-3590 Diepenbeek, Belgium
Keywords: Antioxidative defense system; Arabidopsis thaliana; Gene expression; Oxidative stress; Uranium toxicity
Document URI: http://hdl.handle.net/1942/12066
ISSN: 0265-931X
e-ISSN: 1879-1700
DOI: 10.1016/j.jenvrad.2011.03.015
ISI #: 000292015700013
Category: A1
Type: Journal Contribution
Validations: ecoom 2012
Appears in Collections:Research publications

Show full item record

SCOPUSTM   
Citations

24
checked on Sep 2, 2020

WEB OF SCIENCETM
Citations

28
checked on Dec 4, 2022

Page view(s)

66
checked on Sep 7, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.