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http://hdl.handle.net/1942/12135
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DC Field | Value | Language |
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dc.contributor.author | VERMEEREN, Veronique | - |
dc.contributor.author | GRIETEN, Lars | - |
dc.contributor.author | VANDEN BON, Natalie | - |
dc.contributor.author | BIJNENS, Nathalie | - |
dc.contributor.author | WENMACKERS, Sylvia | - |
dc.contributor.author | JANSSENS, Stoffel | - |
dc.contributor.author | HAENEN, Ken | - |
dc.contributor.author | WAGNER, Patrick | - |
dc.contributor.author | MICHIELS, Luc | - |
dc.date.accessioned | 2011-08-18T14:58:56Z | - |
dc.date.available | NO_RESTRICTION | - |
dc.date.available | 2011-08-18T14:58:56Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | SENSORS AND ACTUATORS B-CHEMICAL, 157(1). p. 130-138 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | http://hdl.handle.net/1942/12135 | - |
dc.description.abstract | The high prevalence of cardiovascular diseases (CVD) demands a reliable and sensitive risk assessment technique. In order to develop a fast and label-free immunosensor for C-reactive protein (CRP), a risk factor for this condition, anti-CRP antibodies were physically adsorbed to the hydrogen (H)-terminated surface of nanocrystalline diamond (NCD). An Enzyme-Linked ImmunoSorbent Assay (ELISA) reference technique showed that this was a suitable substrate for antibody-antigen recognition reactions. Electrochemical Impedance Spectroscopy (EIS) was used to electronically detect CRP recognition. The specificity of the immunosensor was demonstrated by incubation with CRP and plasminogen as reference molecule. A different impedance behavior was observed in real-time after CRP addition as compared to plasminogen addition: the impedance increased only during CRP incubation. Fitting the data showed that this corresponded with a decrease in capacitance of the molecular layer due to its increased thickness by specific CRP recognition. Sensitivity experiments in real-time showed a clear discrimination between 1 mu M,100 nM, and 10 nM of CRP after 10 min at 100 Hz. Since, 10 nM of CRP was still clearly distinguishable from buffer solution, our CRP-directed immunosensor prototype reaches a sensitivity that is within the physiologically relevant concentration range of this biomarker in healthy controls and CVD patients. Moreover, this prototype displayed real-time discriminating power between spiked and unspiked serum, and thus also shows its applicability in this biological matrix. (C) 2011 Elsevier B.V. All rights reserved. | - |
dc.description.sponsorship | The research was performed in the framework of the IAP VI program 'Quantum Effects in Clusters and Nanowires', the Scientific Research Community WOG WO.035.04N 'Hybrid Systems at Nanometer Scale', the Fund for Scientific Research (FWO) projects G.082909 'Synthetic diamond films as platform material for novel DNA sensors with electronic detection principles' and G.0068.07 "Growth, characterisation and simulation of nanocrystalline and ultrananocrystalline PE-CVD diamond films', and the Methusalem project 'NANO Antwerp-Hasselt'. Lars Grieten and Veronique Vermeeren are funded by IWT. We also gratefully acknowledge impulse financing by the transnational University Limburg tUL. Technical assistance by J. Baccus, J. Soogen, and B. Conings in the development of the measurement setups is greatly appreciated. | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.subject.other | Cardiovascular diseases; C-reactive protein; Immunosensor; Impedance spectroscopy; Nanocrystalline diamond; Real-time detection | - |
dc.title | Impedimetric, diamond-based immmunosensor for the detection of C-reactive protein | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 138 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 130 | - |
dc.identifier.volume | 157 | - |
local.format.pages | 9 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Michiels, L (reprint author) [Vermeeren, V; Grieten, L; Vanden Bon, N; Bijnens, N; Wenmackers, S; Janssens, SD; Haenen, K; Wagner, P; Michiels, L] Hasselt Univ, B-3590 Diepenbeek, Belgium [Vermeeren, V; Vanden Bon, N; Michiels, L] Transnat Univ Limburg, Sch Life Sci, Biomed Res Inst, B-3590 Diepenbeek, Belgium [Grieten, L; Bijnens, N; Wenmackers, S; Janssens, SD; Haenen, K; Wagner, P] Transnat Univ Limburg, Sch Life Sci, Inst Mat Res, B-3590 Diepenbeek, Belgium [Haenen, K] IMEC Vzw, Div IMOMEC, B-3590 Diepenbeek, Belgium luc.michiels@uhasselt.be | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1016/j.snb.2011.03.037 | - |
dc.identifier.isi | 000293436900019 | - |
item.contributor | VERMEEREN, Veronique | - |
item.contributor | GRIETEN, Lars | - |
item.contributor | VANDEN BON, Natalie | - |
item.contributor | BIJNENS, Nathalie | - |
item.contributor | WENMACKERS, Sylvia | - |
item.contributor | JANSSENS, Stoffel | - |
item.contributor | HAENEN, Ken | - |
item.contributor | WAGNER, Patrick | - |
item.contributor | MICHIELS, Luc | - |
item.fullcitation | VERMEEREN, Veronique; GRIETEN, Lars; VANDEN BON, Natalie; BIJNENS, Nathalie; WENMACKERS, Sylvia; JANSSENS, Stoffel; HAENEN, Ken; WAGNER, Patrick & MICHIELS, Luc (2011) Impedimetric, diamond-based immmunosensor for the detection of C-reactive protein. In: SENSORS AND ACTUATORS B-CHEMICAL, 157(1). p. 130-138. | - |
item.accessRights | Restricted Access | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2012 | - |
crisitem.journal.eissn | 0925-4005 | - |
Appears in Collections: | Research publications |
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Impedimetric, diamond-based immmunosensor for the detection of C-reactive protein.pdf Restricted Access | Published version | 727.63 kB | Adobe PDF | View/Open Request a copy |
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