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http://hdl.handle.net/1942/12354
Title: | Reusable chromium-coated quartz crystal microbalance for immunosensing | Authors: | Oliver, M. J. Hernando-Garcia, J. POBEDINSKAS, Paulius HAENEN, Ken Rios, A. Sanchez-Rojas, J. L. |
Issue Date: | 2011 | Publisher: | ELSEVIER SCIENCE BV | Source: | COLLOIDS AND SURFACES B-BIOINTERFACES, 88(1). p. 191-195 | Abstract: | The application of oxidized chromium as a reusable platform for the development of immunosensors is presented. Chromium films were deposited on quartz crystal microbalances to study the affinity interaction between rabbit immunoglobulin G (IgG) and goat anti-rabbit IgG. A covalent approach, based on the silane chemistry, was followed for the grafting of either the rabbit IgG or the anti-rabbit IgG on the silane-modified chromium surface. Next the differences between the deposition of rabbit IgG on immobilized anti-rabbit IgG and the deposition of anti-rabbit IgG on immobilized rabbit IgG were investigated. The chromium layer could be reused between experiments, after the proper removal of the organic layers with piranha etch, obtaining a high repeatability in the steps of the functionalization protocol. (C) 2011 Elsevier B.V. All rights reserved. | Notes: | [Oliver, MJ; Hernando-Garcia, J; Sanchez-Rojas, JL] Univ Castilla La Mancha, Dept Ingn Elect Elect Automat & Comunicac, ETSI Ind, E-13071 Ciudad Real, Spain. [Pobedinskas, P; Haenen, K] Hasselt Univ, IMO, B-3590 Diepenbeek, Belgium. [Haenen, K] IMEC VZW, Div IMOMEC, B-3590 Diepenbeek, Belgium. [Rios, A] Univ Castilla La Mancha, Dept Quim Analit & Tecnol Alimentos, Fac Quim, E-13071 Ciudad Real, Spain. MariaJesus.Oliver@uclm.es | Keywords: | Oxidized chromium; Immunosensors; Quartz crystal microbalance | Document URI: | http://hdl.handle.net/1942/12354 | ISSN: | 0927-7765 | e-ISSN: | 1873-4367 | DOI: | 10.1016/j.colsurfb.2011.06.030 | ISI #: | 000295344800025 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2012 |
Appears in Collections: | Research publications |
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