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Title: Molecular diversity of the telson and venom components from Pandinus cavimanus (Scorpionidae Latreille 1802): Transcriptome, venomics and function
Authors: Diego-Garcia, Elia
Peigneur, Steve
Clynen, Elke 
Marien, Tessa
Czech, Lene
Schoofs, Liliane
Tytgat, Jan
Issue Date: 2012
Source: PROTEOMICS, 12(2), p. 313-328
Abstract: Venom from the scorpion Pandinus cavimanus was obtained by electrical stimulation of the telson (stinger). Total venom was toxic to crickets at 730 mu g and a paralysis or lethal effect was observed at 30 mu g of venom (death at 1.5 mu g/mg of cricket). Electrophysiological analyses showed cytolytic activity of total venom on oocytes at 7 mu g. HPLC allowed separation of the venom components. A total of 38 fractions from total venom were tested on voltage-gated Na+ and K+ channels. Some fractions block K+ currents in different degrees. By using MS analysis, we obtained more than 700 different molecular masses from telson and venom fractions (by LC-MS/MS and MALDI-TOF MS analyses). The number of disulfide bridges of the telson components was determined. A cDNA library from P. cavimanus scorpion was constructed and a random sequencing screening of transcripts was conducted. Different clones were obtained and were analyzed by bioinformatics tools. Our results reveal information about new genes related to some cellular processes and genes involved in venom gland functions (toxins, phospholipases and antimicrobial peptides). Expressed sequence tags from venom glands provide complementary information to MS and reveal undescribed components related to the biological activity of the venom.
Notes: [Diego-Garcia, Elia; Peigneur, Steve; Czech, Lene; Tytgat, Jan] Univ Leuven, Toxicol Lab, B-3000 Louvain, Belgium. [Clynen, Elke; Marien, Tessa; Schoofs, Liliane] Univ Leuven, Lab Funct Genom & Prote, B-3000 Louvain, Belgium. [Clynen, Elke] Hasselt Univ, Biomed Res Inst BIOMED, Diepenbeek, Belgium.
Keywords: Biochemical Research Methods; Biochemistry & Molecular Biology; Animal proteomics; Pandinus cavimanus; Scorpion; Transcriptome; Toxin peptide; Venom glands; Venomics;Animal proteomics; Pandinus cavimanus; Scorpion; Transcriptome; Toxin peptide; Venom glands; Venomics
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ISSN: 1615-9853
e-ISSN: 1615-9861
DOI: 10.1002/pmic.201100409
ISI #: 000299153500015
Category: A1
Type: Journal Contribution
Validations: ecoom 2013
Appears in Collections:Research publications

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