Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/13172
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | GRIETEN, Lars | - |
dc.contributor.author | JANSSENS, Stoffel | - |
dc.contributor.author | ETHIRAJAN, Anitha | - |
dc.contributor.author | VANDEN BON, Natalie | - |
dc.contributor.author | AMELOOT, Marcel | - |
dc.contributor.author | MICHIELS, Luc | - |
dc.contributor.author | HAENEN, Ken | - |
dc.contributor.author | WAGNER, Patrick | - |
dc.date.accessioned | 2012-02-22T14:55:13Z | - |
dc.date.available | 2012-02-22T14:55:13Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, 208(9), p. 2093-2098 | - |
dc.identifier.issn | 1862-6300 | - |
dc.identifier.uri | http://hdl.handle.net/1942/13172 | - |
dc.description.abstract | The study of protein adsorption on solid surfaces is interesting for theoretical and practical bio-analytical sensing applications. In this work we combine electrochemical impedance spectroscopy, enzyme linked immunosorbent assay, and fluorescence microscopy with thin boron doped nanocrystalline diamond films to address and study the adsorption behavior of globular proteins (antibodies) on hydrophobic and hydrophilic diamond surfaces. A powerful combination of time resolved impedance spectroscopy and data modeling with equivalent circuits allow a detailed insight in the protein behavior at an interface. It is found that hydrogenated diamond is greatly favorable for impedimetric read-out but causes slight conformational loss of the protein structure and therefore also its biological activity. The oxidized surface allows faster adsorption and a high biological activity but results in smaller impedimetric response | - |
dc.description.sponsorship | In this work we gratefully acknowledge the support of Jan DHaen for the SEM recordings and Johnny Baccus for the technical support. Also the agency for Innovation by Science andtechnlogy (IWT).The researchwas performedinthe framework of the IAP VI program Quantum Effects in Clusters and Nanowires, the Scientific Research Community WOG WO.035.04N Hybrid Systems at Nanometer Scale, the Fund for Scientific Research (FWO) projects G.082909 Synthetic diamond films as platform material for novel DNA sensors with electronic detection principles and G.0068.07 Growth, characterization and simulation of nanocrystalline and ultrananocrystalline PE-CVD diamond films, and the Methusalem project NANO Antwerp-Hasselt. | - |
dc.language.iso | en | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.rights | 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim | - |
dc.subject.other | impedance spectroscopy | - |
dc.subject.other | nanocrystalline diamond | - |
dc.subject.other | protein adsorption | - |
dc.title | Real-time study of protein adsorption on thin nanocrystalline diamond | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 2098 | - |
dc.identifier.issue | 9 | - |
dc.identifier.spage | 2093 | - |
dc.identifier.volume | 208 | - |
local.bibliographicCitation.jcat | A1 | - |
local.publisher.place | POSTFACH 101161, 69451 WEINHEIM, GERMANY | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1002/pssa.201100122 | - |
dc.identifier.isi | 000295433600016 | - |
local.uhasselt.international | no | - |
item.fullcitation | GRIETEN, Lars; JANSSENS, Stoffel; ETHIRAJAN, Anitha; VANDEN BON, Natalie; AMELOOT, Marcel; MICHIELS, Luc; HAENEN, Ken & WAGNER, Patrick (2011) Real-time study of protein adsorption on thin nanocrystalline diamond. In: PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, 208(9), p. 2093-2098. | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2012 | - |
item.contributor | GRIETEN, Lars | - |
item.contributor | JANSSENS, Stoffel | - |
item.contributor | ETHIRAJAN, Anitha | - |
item.contributor | VANDEN BON, Natalie | - |
item.contributor | AMELOOT, Marcel | - |
item.contributor | MICHIELS, Luc | - |
item.contributor | HAENEN, Ken | - |
item.contributor | WAGNER, Patrick | - |
item.accessRights | Restricted Access | - |
crisitem.journal.issn | 1862-6300 | - |
crisitem.journal.eissn | 1862-6319 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Real time study of protein adsorption.pdf Restricted Access | Published version | 473.51 kB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
13
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
14
checked on Sep 27, 2024
Page view(s)
76
checked on Sep 7, 2022
Download(s)
60
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.