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http://hdl.handle.net/1942/13494
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DC Field | Value | Language |
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dc.contributor.author | BUNTINX, Mieke | - |
dc.contributor.author | AMELOOT, Marcel | - |
dc.contributor.author | STEELS, Paul | - |
dc.contributor.author | JANSSEN, Paul | - |
dc.contributor.author | MEDAER, Rob | - |
dc.contributor.author | GEUSENS, Piet | - |
dc.contributor.author | RAUS, Jef | - |
dc.contributor.author | STINISSEN, Piet | - |
dc.date.accessioned | 2012-03-29T13:23:09Z | - |
dc.date.available | 2012-03-29T13:23:09Z | - |
dc.date.issued | 2001 | - |
dc.identifier.citation | Archives of Physiology and Biochemistry, 113, p. B5-B5 | - |
dc.identifier.issn | 1381-3455 | - |
dc.identifier.uri | http://hdl.handle.net/1942/13494 | - |
dc.description.abstract | Autoreactive T lymphocytes are considered to play a crucial role in orchestrating an immunopathological cascade that culminates in damage to the myelin sheath, oligodendrocytes and axons in the central nervous system (CNS) of multiple sclerosis (MS) patients. Autoreactive T cells may also initiate a chronic inflammation in the joints of rheumatoid arthritis (RA) patients. However, autoreactive T cells appear to be only a small component of this immune infiltrate. It has been suggested that the majority of T cells are nonspecifically recruited into the CNS of MS patients and into the inflamed joint of RA patients. The mechanisms underlying the peripheral activation of non-autoantigen specific T cells in MS and RA and their clinical relevance remain however largely unknown. Several lines of evidence indicate that interferon-gamma (IFN-g) may play an important role in the pathogenesis of MS and RA. Here, we have studied whether T cells from patients with autoimmune diseases are more susceptible to T cell activation in the presence of IFN-g. We studied the effects of IFN-g on Ca2+-release from intracellular stores and Ca2+-influx from the extracellular environment in peripheral blood T cells from MS and RA patients, patients with common viral infections (flu-like symptoms) and healthy donors. Fluorescence imaging microscopy was used to monitor the intracellular [Ca2+]i signal in individual T cells. Our results indicate that IFN-g mediates a sustained elevated [Ca2+]i in T cells of (active) MS and RA patients as compared to healthy controls and flu patients. Furthermore, no [Ca2+]i increase was observed in Ca2+-free medium, excluding an effect of IFN-g on Ca2+-release from intracellular stores. Although this IFN-g-activated Ca2+-influx seems to be insufficient to induce T cell proliferation in vitro, our data indicate that proliferation in response to suboptimal doses of phytohemagglutine can significantly be augmented in the presence of IFN-g. This study suggests that the Ca2+-influx stimulated by IFN-g can act as a complementary mechanism in the activation of non-autoantigen specific T cells in the blood of patients affected by autoimmune disorders as MS and RA. | - |
dc.language.iso | en | - |
dc.title | Interferon-gamma-induced Ca2+-influx in T lymphocytes of Multiple Sclerosis and Rheumatoid Arthritis patients: A complementary mechanism for T cell activation? | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | B5 | - |
dc.identifier.spage | B5 | - |
dc.identifier.volume | 113 | - |
local.bibliographicCitation.jcat | A3 | - |
local.type.refereed | Non-Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A2 | - |
item.contributor | BUNTINX, Mieke | - |
item.contributor | AMELOOT, Marcel | - |
item.contributor | STEELS, Paul | - |
item.contributor | JANSSEN, Paul | - |
item.contributor | MEDAER, Rob | - |
item.contributor | GEUSENS, Piet | - |
item.contributor | RAUS, Jef | - |
item.contributor | STINISSEN, Piet | - |
item.accessRights | Closed Access | - |
item.fullcitation | BUNTINX, Mieke; AMELOOT, Marcel; STEELS, Paul; JANSSEN, Paul; MEDAER, Rob; GEUSENS, Piet; RAUS, Jef & STINISSEN, Piet (2001) Interferon-gamma-induced Ca2+-influx in T lymphocytes of Multiple Sclerosis and Rheumatoid Arthritis patients: A complementary mechanism for T cell activation?. In: Archives of Physiology and Biochemistry, 113, p. B5-B5. | - |
item.fulltext | No Fulltext | - |
crisitem.journal.issn | 1381-3455 | - |
crisitem.journal.eissn | 1744-4160 | - |
Appears in Collections: | Research publications |
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