Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/13498
Title: | Liver X receptors regulate cholesterol homeostasis in oligodendrocytes | Authors: | NELISSEN, Katherine Mulder, Monique SMETS, Ilse TIMMERMANS, Silke SMEETS, Karen AMELOOT, Marcel HENDRIKS, Jerome |
Issue Date: | 2012 | Publisher: | WILEY-BLACKWELL | Source: | JOURNAL OF NEUROSCIENCE RESEARCH, 90 (1), p. 60-71 | Abstract: | Cholesterol synthesis and transport in oligodendrocytes are essential for optimal myelination and remyelination in pathological conditions such as multiple sclerosis. However, little is known about cholesterol homeostasis in the myelin-forming oligodendrocytes. Liver X receptors (LXRs) are nuclear oxysterol receptors that regulate genes involved in cholesterol homeostasis and may therefore play an important role in de- and remyelination. We investigated whether LXRs regulate cholesterol homeostasis in oligodendrocytes. mRNA expression of genes encoding LXR-alpha and LXR-beta and their target genes (ABCA1, ABCG1, ABCG4, apoE, and LDLR) was detected in oligodendrocytes derived from both neonatal and adult rats using quantitative real-time PCR. The expression of LXR-beta and several target genes was increased during oligodendrocyte differentiation. We further demonstrated that treatment of primary neonatal rat oligodendrocytes with the synthetic LXR agonist T0901317 induced the expression of several established LXR target genes, including ABCA1, ABCG1, apoE, and LDLR. Treatment of oligodendrocytes with T0901317 resulted in an enhanced cholesterol efflux in the presence of apolipoprotein A-I or high-density lipoprotein particles. These data show that LXRs are involved in regulating cholesterol homeostasis in oligodendrocytes. | Notes: | 000297951100006, [Nelissen, Katherine; Timmermans, Silke; Ameloot, Marcel; Hendriks, Jerome J. A.] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [Nelissen, Katherine; Timmermans, Silke; Ameloot, Marcel; Hendriks, Jerome J. A.] Transnat Univ Limburg, Diepenbeek, Belgium. [Mulder, Monique] Rotterdam Univ, Div Pharmacol Vasc & Metab Dis, Dept Internal Med, Rotterdam, Netherlands. [Smets, Ilse] PHL Univ Coll, Dept PHL Bio, Diepenbeek, Belgium. [Smeets, Karen] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. Jerome.hendriks@uhasselt.be | Keywords: | ATP-binding cassette transporters; apolipoprotein E; low-density lipoprotein receptor; qPCR;neurosciences | Document URI: | http://hdl.handle.net/1942/13498 | ISSN: | 0360-4012 | e-ISSN: | 1097-4547 | DOI: | 10.1002/jnr.22743 | ISI #: | 000297951100006 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2013 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
liver x receptors.pdf Restricted Access | Published version | 1.47 MB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
36
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
59
checked on Oct 14, 2024
Page view(s)
68
checked on Sep 7, 2022
Download(s)
50
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.