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Title: Unchanged expression of the ceramide transfer protein in the acute 6-OHDA neurodegenerative model
Authors: Mencarelli, Chiara
Bode, Gerard H.
Vlamings, Rinske
Janssen, Marcus L. F.
Losen, Mario
Steinbusch, Harry W. M.
Temel, Yasin
Martinez-Martinez, Pilar
Issue Date: 2012
Source: NEUROSCIENCE LETTERS, 506 (1), p. 39-43
Abstract: Ceramides are lipids that are abundant in brain tissue where they have an important structural role in cellular membranes. Ceramides are also powerful intracellular signalling molecules controlling cell death, growth and differentiation. So far, the ceramide transfer protein (CERT), a shorter splice variant of the Goodpasture antigen-binding protein (GPBP), is the only known protein with the ability to shuttle ceramide from the endoplasmic reticulum to the Golgi apparatus. GPBP/CERT are widely distributed in the central nervous system where they act as key factors for normal brain development and homeostasis. Ceramide accumulates in neurons during acute neurodegeneration. The objective of this study was to define whether levels of the ceramide transfer protein GPBP/CERT are altered in the acute neurodegenerative process. We used design-based stereology to quantify the number of GPBP/CERT immunoreactive cells in the striatum of 6-hydroxydopamine (6-OHDA) lesioned rats as an animal model of Parkinson's disease (PD). In addition, gray value measurement was performed to quantify GPBP/CERT immunoreactivity-levels within individual cells. No difference in the striatal expression levels of GPBP/CERT proteins was found between diseased and control animals, suggesting that the expression pattern of GPBP/CERT in the striatum is not affected in the 6-OHDA rat model of PD. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
Notes: Martinez-Martinez, P (reprint author), Maastricht Univ, Fac Hlth Med & Life Sci, Dept Neurosci, POB 616, NL-6200 MD Maastricht, Netherlands.
Keywords: Parkinsons's disease(PD); Ceramide transporter (CERT); Goodpasture-antigen binding protein(GPBP); 6-hydroxydopamine(6-OHDA); Ceramide; Neurostereology
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ISSN: 0304-3940
e-ISSN: 1872-7972
DOI: 10.1016/j.neulet.2011.10.044
ISI #: 000300205000008
Category: A1
Type: Journal Contribution
Validations: ecoom 2013
Appears in Collections:Research publications

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