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|Title:||Interleukin-1 beta and neurotrophin-3 synergistically promote neurite growth in vitro||Authors:||Boato, Francesco
Peters, Eva M.
|Issue Date:||2011||Publisher:||BIOMED CENTRAL LTD||Source:||JOURNAL OF NEUROINFLAMMATION, 8 (Article 183)||Abstract:||Pro-inflammatory cytokines such as interleukin-1 beta (IL-1 beta) are considered to exert detrimental effects during brain trauma and in neurodegenerative disorders. Consistently, it has been demonstrated that IL-1 beta suppresses neurotrophin-mediated neuronal cell survival rendering neurons vulnerable to degeneration. Since neurotrophins are also well known to strongly influence axonal plasticity, we investigated here whether IL-1 beta has a similar negative impact on neurite growth. We analyzed neurite density and length of organotypic brain and spinal cord slice cultures under the influence of the neurotrophins NGF, BDNF, NT-3 and NT-4. In brain slices, only NT-3 significantly promoted neurite density and length. Surprisingly, a similar increase of neurite growth was induced by IL-1 beta. Additionally, both factors increased the number of brain slices displaying maximal neurite growth. Furthermore, the co-administration of IL-1 beta and NT-3 significantly increased the number of brain slices displaying maximal neurite growth compared to single treatments. These data indicate that these two factors synergistically stimulate two distinct aspects of neurite outgrowth, namely neurite density and neurite length from acute organotypic brain slices.||Notes:||[Boato, Francesco; Hendrix, Sven] Hasselt Univ, Dept Funct Morphol, Hasselt, Belgium. [Boato, Francesco; Hendrix, Sven] Hasselt Univ, BIOMED Inst, Hasselt, Belgium. [Hechler, Daniel; Rosenberger, Karen; Luedecke, Doreen] Charite Univ Med Berlin, Ctr Anat, Inst Cell Biol & Neurobiol, Berlin, Germany. [Peters, Eva M.] Charite, Charite Ctr Internal Med & Dermatol 12, D-10117 Berlin, Germany. [Peters, Eva M.] Univ Giessen, Dept Psychosomat Med, Giessen, Germany. [Nitsch, Robert] Johannes Gutenberg Univ Mainz, Univ Med Mainz, Inst Microscop Anat & Neurobiol, Mainz, Germany. email@example.com||Keywords:||interleukin 1 beta; IL-1 beta; neurotrophin 3; NT-3; NGF; spinal cord; brain slices; neurite growth; axon outgrowth; neuroplasticity;interleukin 1 beta; IL-1 beta; neurotrophin 3; NT-3; NGF; spinal cord; brain slices; neurite growth; axon outgrowth; neuroplasticity||Document URI:||http://hdl.handle.net/1942/13500||e-ISSN:||1742-2094||DOI:||10.1186/1742-2094-8-183||ISI #:||000300342000001||Category:||A1||Type:||Journal Contribution||Validations:||ecoom 2013|
|Appears in Collections:||Research publications|
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