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http://hdl.handle.net/1942/13594
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DC Field | Value | Language |
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dc.contributor.author | Loske, Peter | - |
dc.contributor.author | Boato, Francesco | - |
dc.contributor.author | HENDRIX, Sven | - |
dc.contributor.author | Piepgras, Johannes | - |
dc.contributor.author | Just, Ingo | - |
dc.contributor.author | Ahnert-Hilger, Gudrun | - |
dc.contributor.author | Hoeltje, Markus | - |
dc.date.accessioned | 2012-04-27T11:35:44Z | - |
dc.date.available | 2012-04-27T11:35:44Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | JOURNAL OF NEUROCHEMISTRY, 120 (6), p. 1084-1096 | - |
dc.identifier.issn | 0022-3042 | - |
dc.identifier.uri | http://hdl.handle.net/1942/13594 | - |
dc.description.abstract | C3 ADP-ribosyltransferase is a valuable tool to study Rho-dependent cellular processes. In the current study we investigated the impact of enzyme-deficient peptides derived from Clostridium botulinum C3 transferase in the context of neuronal process elongation and branching, synaptic connectivity, and putative beneficial effects on functional outcome following traumatic injury to the CNS. By screening a range of peptidic fragments, we identified three short peptides from C3bot that promoted axon and dendrite outgrowth in cultivated hippocampal neurons. Furthermore, one of these fragments, a 26-amino acid peptide covering the residues 156181 enhanced synaptic connectivity in primary hippocampal culture. This peptide was also effective to foster axon outgrowth and re-innervation in organotypical brain slice culture. To evaluate the potential of the 26mer to foster repair mechanisms after CNS injury we applied this peptide to mice subjected to spinal cord injury by either compression impact or hemisection. A single local administration at the site of the lesion improved locomotor recovery. In addition, histological analysis revealed an increased serotonergic input to lumbar motoneurons in treated compared with control mice. Pull-down assays showed that lesion-induced up-regulation of RhoA activity within the spinal cord was largely blocked by C3bot peptides despite the lack of enzymatic activity. | - |
dc.description.sponsorship | The authors are indebted to Birgit Metze, Annemarie Lo¨chner and Julia Ko¨nig for their engaged and skillful technical assistance. Work was supported by grants from the Deutsche Forschungsgemeinschaft (DFG) to MH and GAH (HO 3249/2–1), IJ (JU 231/5–1) and SH (SPP1394). This study was also supported in part by grants from the Berlin-Brandenburg Center for Regenerative Therapies and the Flemish Research Council (FWO) to SH. All authors declare no conflicts of interest. | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.rights | 2012 International Society for Neurochemistry | - |
dc.subject.other | C3 transferasere | - |
dc.subject.other | generation | - |
dc.subject.other | RhoA | - |
dc.subject.other | spinal cord injury | - |
dc.title | Minimal essential length of Clostridium botulinum C3 peptides to enhance neuronal regenerative growth and connectivity in a non-enzymatic mode | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 1096 | - |
dc.identifier.issue | 6 | - |
dc.identifier.spage | 1084 | - |
dc.identifier.volume | 120 | - |
local.format.pages | 13 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Hoeltje, Markus] Charite, Inst Integrat Neuroanat, Ctr Anat, D-10115 Berlin, Germany. [Boato, Francesco; Hendrix, Sven] Hasselt Univ, Dept Morphol, Hasselt, Belgium. [Boato, Francesco; Hendrix, Sven] Hasselt Univ, Inst Biomed, Hasselt, Belgium. [Just, Ingo] Hannover Med Sch MHH, Inst Toxicol, Hannover, Germany. | - |
local.publisher.place | MALDEN | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1111/j.1471-4159.2012.07657.x | - |
dc.identifier.isi | 000301112500020 | - |
dc.identifier.eissn | 1471-4159 | - |
local.uhasselt.international | yes | - |
item.contributor | Loske, Peter | - |
item.contributor | Boato, Francesco | - |
item.contributor | HENDRIX, Sven | - |
item.contributor | Piepgras, Johannes | - |
item.contributor | Just, Ingo | - |
item.contributor | Ahnert-Hilger, Gudrun | - |
item.contributor | Hoeltje, Markus | - |
item.accessRights | Restricted Access | - |
item.fullcitation | Loske, Peter; Boato, Francesco; HENDRIX, Sven; Piepgras, Johannes; Just, Ingo; Ahnert-Hilger, Gudrun & Hoeltje, Markus (2012) Minimal essential length of Clostridium botulinum C3 peptides to enhance neuronal regenerative growth and connectivity in a non-enzymatic mode. In: JOURNAL OF NEUROCHEMISTRY, 120 (6), p. 1084-1096. | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2013 | - |
crisitem.journal.issn | 0022-3042 | - |
crisitem.journal.eissn | 1471-4159 | - |
Appears in Collections: | Research publications |
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loske 1.pdf Restricted Access | Published version | 967.23 kB | Adobe PDF | View/Open Request a copy |
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