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http://hdl.handle.net/1942/13599
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DC Field | Value | Language |
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dc.contributor.author | Finoulst, Inez | - |
dc.contributor.author | Pinkse, Martijn | - |
dc.contributor.author | Van Dongen, William | - |
dc.contributor.author | VERHAERT, Peter | - |
dc.date.accessioned | 2012-04-27T12:25:50Z | - |
dc.date.available | 2012-04-27T12:25:50Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2011 (Article ID 245291) | - |
dc.identifier.issn | 1110-7243 | - |
dc.identifier.uri | http://hdl.handle.net/1942/13599 | - |
dc.description.abstract | Although big progress has been made in sample pretreatment over the last years, there are still considerable limitations when it comes to overcoming complexity and dynamic range problems associated with peptide analyses from biological matrices. Being the little brother of proteomics, peptidomics is a relatively new field of research aiming at the direct analysis of the small proteins, called peptides, many of which are not amenable for typical trypsin-based analytics. In this paper, we present an overview of different techniques and methods currently used for reducing a sample's complexity and for concentrating low abundant compounds to enable successful peptidome analysis. We focus on techniques which can be employed prior to liquid chromatography coupled to mass spectrometry for peptide detection and identification and indicate their advantages as well as their shortcomings when it comes to the untargeted analysis of native peptides from complex biological matrices. | - |
dc.language.iso | en | - |
dc.publisher | HINDAWI PUBLISHING CORPORATION | - |
dc.subject.other | Biotechnology & Applied Microbiology; Medicine; Research & Experimental | - |
dc.title | Sample Preparation Techniques for the Untargeted LC-MS-Based Discovery of Peptides in Complex Biological Matrices | - |
dc.type | Journal Contribution | - |
dc.identifier.issue | Article ID 245291 | - |
dc.identifier.volume | 2011 | - |
local.format.pages | 14 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Finoulst, Inez; Pinkse, Martijn; Van Dongen, William; Verhaert, Peter] Delft Univ Technol, Dept Biotechnol, Lab Analyt Biotechnol & Innovat Peptide Biol, NL-3706 AV Zeist, Netherlands. [Pinkse, Martijn; Verhaert, Peter] Delft Univ Technol, Netherlands Prote Ctr, NL-3706 AV Zeist, Netherlands. [Van Dongen, William] TNO Triskel, NL-3700 AV Zeist, Netherlands. [Verhaert, Peter] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. | - |
local.publisher.place | NEW YORK | - |
local.type.refereed | Refereed | - |
local.type.specified | Review | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1155/2011/245291 | - |
dc.identifier.isi | 000298684700001 | - |
item.fulltext | With Fulltext | - |
item.contributor | Finoulst, Inez | - |
item.contributor | Pinkse, Martijn | - |
item.contributor | Van Dongen, William | - |
item.contributor | VERHAERT, Peter | - |
item.accessRights | Open Access | - |
item.fullcitation | Finoulst, Inez; Pinkse, Martijn; Van Dongen, William & VERHAERT, Peter (2011) Sample Preparation Techniques for the Untargeted LC-MS-Based Discovery of Peptides in Complex Biological Matrices. In: JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2011 (Article ID 245291). | - |
item.validation | ecoom 2013 | - |
crisitem.journal.issn | 1110-7243 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Finoulst-sample preparation techniques.pdf | 1.05 MB | Adobe PDF | View/Open |
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