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Title: Goodpasture Antigen-binding Protein/Ceramide Transporter Binds to Human Serum Amyloid P-Component and Is Present in Brain Amyloid Plaques
Authors: Mencarelli, Chiara
Bode, Gerard H.
Losen, Mario
Kulharia, Mahesh
Molenaar, Peter C.
Veerhuis, Robert
Steinbusch, Harry W. M.
De Baets, Marc H. 
Nicolaes, Gerry A. F.
Martinez-Martinez, Pilar
Issue Date: 2012
Source: JOURNAL OF BIOLOGICAL CHEMISTRY, 287 (18), p. 14897-14911
Abstract: Serum amyloid P component (SAP) is a non-fibrillar glycoprotein belonging to the pentraxin family of the innate immune system. SAP is present in plasma, basement membranes, and amyloid deposits. This study demonstrates, for the first time, that the Goodpasture antigen-binding protein (GPBP) binds to human SAP. GPBP is a nonconventional Ser/Thr kinase for basement membrane type IV collagen. Also GPBP is found in plasma and in the extracellular matrix. In the present study, we demonstrate that GPBP specifically binds SAP in its physiological conformations, pentamers and decamers. The START domain in GPBP is important for this interaction. SAP and GPBP form complexes in blood and partly colocalize in amyloid plaques from Alzheimer disease patients. These data suggest the existence of complexes of SAP and GPBP under physiological and pathological conditions. These complexes are important for understanding basement membrane, blood physiology, and plaque formation in Alzheimer disease.
Notes: [Mencarelli, Chiara; Bode, Gerard H.; Losen, Mario; Molenaar, Peter C.; Steinbusch, Harry W. M.; De Baets, Marc H.; Martinez-Martinez, Pilar] Maastricht Univ, Dept Neurosci, Sch Mental Hlth & Neurosci, NL-6200 MD Maastricht, Netherlands. [Kulharia, Mahesh; Nicolaes, Gerry A. F.] Maastricht Univ, Dept Biochem, Cardiovasc Res Inst Maastricht, NL-6200 MD Maastricht, Netherlands. [Veerhuis, Robert] Vrije Univ Amsterdam, Dept Clin Chem, Univ Med Ctr, NL-1007 MB Amsterdam, Netherlands. [Veerhuis, Robert] Vrije Univ Amsterdam, Dept Psychiat, Univ Med Ctr, NL-1007 MB Amsterdam, Netherlands. [Veerhuis, Robert] Vrije Univ Amsterdam, Alzheimer Ctr, Univ Med Ctr, NL-1007 MB Amsterdam, Netherlands. [De Baets, Marc H.] Hasselt Univ, Neuroimmunol Grp, Biomed Res Inst BIOMED, Diepenbeek, Belgium.
Keywords: Biochemistry & Molecular Biology
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ISSN: 0021-9258
e-ISSN: 1083-351X
DOI: 10.1074/jbc.M111.299545
ISI #: 000304003200053
Category: A1
Type: Journal Contribution
Validations: ecoom 2013
Appears in Collections:Research publications

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