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Title: Exploring and comparing the angiogenic properties of different dental stem cell populations
Authors: Fanton, Yanick
Advisors: LAMBRICHTS, Ivo
Issue Date: 2012
Publisher: tUL Diepenbeek
Abstract: Therapeutic angiogenesis is very promising, however, current revascularization strategies are unsuccessful. Since angiogenesis is key to the development of the tooth, this study compared the angiogenic potential of 3 dental stem cell (DSC) types: dental pulp stem cells (DPSCs), dental follicle stem cells (FSCs) and stem cells from the apical papilla (SCAPs). Angiogenic factors secreted by the DSCs were identified in a Human Angiogenesis Array, followed by validation by means of ELISA and RT-PCR. In vitro assays with a Human Microvascular Endothelial Cell line (HMEC-1) were performed to gain more insights in the effect of the DSCs on HMEC-1 proliferation, migration and tube formation in an MTT assay, a transwell system and a Matrigel assay respectively. The various DSCs express pro- and anti-angiogenic factors and induce HMEC-1 migration and tube formation, but not proliferation. DSCs thus provide opportunities to treat patients with cells from their own or other individuals' teeth.
Notes: master in de biomedische wetenschappen-klinische moleculaire wetenschappen
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Category: T2
Type: Theses and Dissertations
Appears in Collections:Master theses

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