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Title: Cell type-associated differences in migration, survival and immunogenicity following grafting in CNS tissue
Authors: Praet, J.
Reekmans, K.
LIN, Dan 
De Vocht, N.
Daans, J.
HENS, Niel 
Pauwels, P.
Berneman, Z.
Van der Linden, A.
Ponsaerts, P.
Issue Date: 2012
Source: JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 6 (Special Issue, Supplement 1), p. 270-270
Abstract: Cell transplantation has been suggested to display several neuro-protective and/or - regenerative effects in animal models of central nervous system (CNS) trauma. However, while most studies report on clinical observations, currently little is known regarding the actual fate of the cell populations grafted and whether or how the brain's innate immune system, mainly directed by activated microglia and astrocytes, interacts with autologous cellular implants. In this study, we grafted well-characterised neural stem cell, mouse embryonic fibroblast, dendritic cell, bone marrow mononuclear cell and splenocyte populations, all isolated or culturedfrom C57BL/6-eGFP transgenic mice, below the capsula externa (CE) of healthy C57BL/6 mice and below the inflamed/demyelinated CE of cuprizone-treated C57BL/6 mice. Two weeks post-grafting, an extensive quantitative multicolour histological analysis was performed in order: (i) to quantify cell graft localisation, migration, survival and toxicity, and (ii) to characterize endogenous CNS immune responses against the different cell grafts. Obtained results indicate dependent on the cell type grafted: (i) a different degree of cell graft migration, survival and toxicity, and (ii) a different organisation of the endogenous immune response. Based on these observations, we warrant that further research should be taken to understand - and eventually control - cell graft induced tissue damage and activation of the brain's innate immune system. The latte will be inevitable before cell grafting in the CNS can be performed safely and succesfully in clinical settings.
Notes: Univ Antwerp, Antwerp, Belgium. Univ Hasselt, Hasselt, Belgium.
Keywords: Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Cell Biology; Engineering, Biomedical
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ISSN: 1932-6254
e-ISSN: 1932-7005
ISI #: 000308313002194
Category: M
Type: Journal Contribution
Appears in Collections:Research publications

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