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Title: Resveratrol-Induced Changes of the Human Adipocyte Secretion Profile
Authors: Rosenow, Anja
NOBEN, Jean-Paul 
Jocken, Johan
Kallendrusch, Sonja
Fischer-Posovszky, Pamela
Mariman, Edwin C. M.
Renes, Johan
Issue Date: 2012
Source: JOURNAL OF PROTEOME RESEARCH, 11 (9), p. 4733-4743
Abstract: Enlarged white adipose tissue (WAT) is a feature of obesity and leads to changes in its paracrine and endocrine function. Dysfunction of WAT cells is associated with obesity-associated disorders like type 2 diabetes and cardiovascular diseases. Resveratrol (RSV), a natural polyphenolic compound, mimics beneficial effects of calorie restriction. As such, RSV seems a promising therapeutic target for obesity-associated disorders. The effect of RSV on the human adipokine profile is still elusive. Therefore, a proteomic study together with bioinformatical analysis was performed to investigate the effect of RSV on the secretion profile of mature human SGBS adipocytes. RSV incubation resulted in elevated basal glycerol release and reduced intracellular TG content. This increased intracellular lipolysis was accompanied by profound changes in the adipocyte secretion profile. Extracellular matrix proteins were down-regulated while processing proteins were mostly up-regulated after RSV treatment. Interestingly, RSV induced secretion of proteins protective against cellular stress and proteins involved in the regulation of apoptosis. Furthermore, we found a RSV-induced up-regulation of adiponectin and ApoE accompanied by a down-regulation of PAI-1 and PEDF secretion which may improve anti-inflammatory processes and increased insulin sensitivity. These effects may contribute to alleviate obesity-induced metabolic complications. In addition, two novel RSV-regulated adipocyte-secreted proteins were identified.
Notes: [Rosenow, Anja; Jocken, Johan; Kallendrusch, Sonja; Mariman, Edwin C. M.; Renes, Johan] Maastricht Univ, Dept Human Biol, NUTRIM Sch Nutr Toxicol & Metab, NL-6200 MD Maastricht, Netherlands. [Noben, Jean-Paul] Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium. [Noben, Jean-Paul] Transnat Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. [Fischer-Posovszky, Pamela] Univ Ulm, Med Ctr, Div Pediat Endocrinol & Diabet, Endocrine Res Lab, D-89069 Ulm, Germany.
Keywords: human adipocytes; RSV; adipokines; lipolysis; 2-DE LC-MS/MS;Biochemical Research Methods; human adipocytes; RSV; adipokines; lipolysis; 2-DE LC MS/MS
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ISSN: 1535-3893
e-ISSN: 1535-3907
DOI: 10.1021/pr300539b
ISI #: 000308389900031
Category: A1
Type: Journal Contribution
Validations: ecoom 2013
Appears in Collections:Research publications

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