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Title: Sampling Site Matters When Counting Lymphocyte Subpopulations
Authors: OGUNJIMI, Benson 
Peeters, Dieter
HENS, Niel 
Malfait, Ronald
Van Tendeloo, Viggo
Van Damme, Pierre
Beutels, Philippe
Smits, Evelien
Issue Date: 2012
Source: PLOS ONE, 7 (7), p. e41405
Abstract: Clinical and scientific work routinely relies on antecubital venipunctures for hematological, immunological or other analyses on blood. This study tested the hypothesis that antecubital veins can be considered to be a good proxy for other sampling sites. Using a hematocytometer and a flow cytometer, we analyzed the cell counts from samples coming from the radial artery, the dorsal hand veins and the antecubital veins from 18 volunteers. Most surprisingly, we identified the greatest difference not to exist between arterial and venous circulation, but between the distal (radial artery & dorsal hand veins) and proximal (antecubital veins) sampling sites. Naive T cells had a higher cell count distally compared to proximally and the reverse was true for effector memory T cells. Despite these differences there were high correlations between the different sampling sites, which partially supports our initial hypothesis. Our findings are crucial for the future design and interpretation of immunological research, and for clinical practice. Furthermore, our results suggest a role for interval lymph nodes in the trafficking of lymphocytes.
Notes: [Ogunjimi, Benson; Hens, Niel; Beutels, Philippe] Univ Antwerp, Vaccine & Infect Dis Inst, Ctr Hlth Econ Res & Modeling Infect Dis, B-2020 Antwerp, Belgium. [Peeters, Dieter] GZA Hosp Sint Augustinus, Ctr Oncol, Translat Canc Res Unit, Antwerp, Belgium. [Peeters, Dieter] Univ Antwerp, Dept Med Oncol, B-2020 Antwerp, Belgium. [Malfait, Ronald] Univ Antwerp Hosp, Dept Lab Med, Antwerp, Belgium. [Hens, Niel] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Hasselt, Belgium. [Van Tendeloo, Viggo; Smits, Evelien] Univ Antwerp, Vaccine & Infect Dis Inst, Lab Expt Hematol, B-2020 Antwerp, Belgium. [Van Tendeloo, Viggo; Smits, Evelien] Univ Antwerp Hosp, Ctr Cell Therapy & Regenerat Med, Antwerp, Belgium. [Van Damme, Pierre] Univ Antwerp, Vaccine & Infect Dis Inst, Ctr Evaluat Vaccinat, B-2020 Antwerp, Belgium. [Beutels, Philippe] Univ New S Wales, Sch Publ Hlth & Community Med, Sydney, NSW, Australia.
Keywords: Multidisciplinary Sciences
Document URI:
ISSN: 1932-6203
e-ISSN: 1932-6203
DOI: 10.1371/journal.pone.0041405
ISI #: 000306806600062
Category: A1
Type: Journal Contribution
Validations: ecoom 2013
Appears in Collections:Research publications

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