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Title: Expression Pattern of Basal Markers in Human Dental Pulp Stem Cells and Tissue
Authors: MARTENS, Wendy 
WOLFS, Esther
POLITIS, Constantinus 
Issue Date: 2012
Publisher: KARGER
Source: CELLS TISSUES ORGANS, 196 (6), p. 490-500
Abstract: Dental pulp stem cells (DPSC) have been characterized as a multipotent stem cell population, with the ability to differentiate into mesodermal and neural cell lineages. Although 'de novo' expression of neural markers after differentiation is mostly considered as proof of differentiation, expression of these markers in undifferentiated DPSC is not well described. Therefore, an immunocytochemical analysis was performed to evaluate the neural marker expression of undifferentiated human DPSC (hDPSC) in in vitro cultures. Undifferentiated hDPSC uniformly expressed neural markers beta-III-tubulin, S100 protein and synaptophysin. A subset of the population showed a positive immune-reactivity for galactocerebroside, neurofilament and nerve growth factor receptor p75. Furthermore, the location of possible stem cell niches, present in young dental pulp tissue, was determined by means of immunohistochemistry based on mesenchymal and neural marker expression. The results demonstrated the presence of a perivascular niche and a second stem cell niche at the cervical area. In adult dental pulp, only a perivascular niche could be observed. Based on the expression of neural markers in naive DPSC, it has to be taken into account that not only the marker expression upon neural differentiation must be analyzed, but an ultrastructural analysis of the morphological changes and functional studies must also be performed to confirm a successful differentiation.
Notes: Martens, W (reprint author), [Martens, W.; Struys, T.; Politis, C.; Bronckaers, A.; Lambrichts, I.] Hasselt Univ, Histol Lab, Biomed Res Inst, BE-3590 Diepenbeek, Belgium. [Wolfs, E.] Katholieke Univ Leuven, Dept Med Diagnost Sci, Div Nucl Med, Louvain, Belgium. [Politis, C.] Hosp E Limburg, Dept Maxillofacial Surg, Genk, Belgium.
Keywords: Anatomy & Morphology; Cell Biology; Developmental Biology; dental pulp stem cells; mesenchymal markers; neural markers; stem cell niche;Dental pulp stem cells; Mesenchymal markers; Neural markers; Stem cell niche
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ISSN: 1422-6405
e-ISSN: 1422-6421
DOI: 10.1159/000338654
ISI #: 000311470700002
Category: A1
Type: Journal Contribution
Validations: ecoom 2013
Appears in Collections:Research publications

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