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Title: Complex invasion pattern of the cerebral cortex bymicroglial cells during development of the mouse embryo
Authors: Swinnen, Nina 
Smolders, Sophie 
Avila, Ariel 
Notelaers, Kristof 
Paesen, Rik 
Ameloot, Marcel 
Brone, Bert 
Legendre, Pascal
Rigo, Jean-Michel 
Issue Date: 2013
Source: GLIA, 61 (2), p. 150-163
Abstract: Microglia are the immune cells of the central nervous system. They are suspected to play important roles in adult synaptogenesis and in the development of the neuronal network. Microglial cells originate from progenitors in the yolk sac. Although it was suggested that they invade the cortex at early developmental stages in the embryo, their invasion pattern remains largely unknown. To address this issue we analyzed the pattern of cortical invasion by microglial cells in mouse embryos at the onset of neuronal cell migration using in vivo immunohistochemistry and ex vivo time-lapse analysis of microglial cells. Microglial cells begin to invade the cortex at 11.5 days of embryonic age (E11.5). They first accumulate at the pial surface and within the lateral ventricles, after which they spread throughout the cortical wall, avoiding the cortical plate region in later embryonic ages. The invasion of the cortical parenchyma occurs in different phases. First, there is a gradual increase of microglial cells between E10.5 and E14.5. From E14.5 to E15.5 there is a rapid phase with a massive increase in microglia, followed by a slow phase again from E15.5 until E17.5. At early stages, many peripheral microglia are actively proliferating before entering the parenchyma. Remarkably, activated microglia accumulate in the choroid plexus primordium, where they are in the proximity of dying cells. Time-lapse analysis shows that embryonic microglia are highly dynamic cells. (c) 2012 Wiley Periodicals, Inc.
Notes: [Swinnen, Nina; Smolders, Sophie; Avila, Ariel; Notelaers, Kristof; Paesen, Rik; Ameloot, Marcel; Brone, Bert; Rigo, Jean-Michel] Hasselt Univ, BIOMED, B-3590 Diepenbeek, Belgium. [Swinnen, Nina; Legendre, Pascal] Univ Paris 06, INSERM, U952, Paris, Ile De France, France. [Swinnen, Nina; Legendre, Pascal] Univ Paris 06, CNRS, UMR 7224, Paris, Ile De France, France. [Notelaers, Kristof] Katholieke Univ Leuven, Lab Photochem & Spect, Dept Chem, Louvain, Belgium.
Keywords: Neurosciences; microglia; embryo; cortex; choroid plexus;microglia; embryo; cortex; choroid plexus
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ISSN: 0894-1491
e-ISSN: 1098-1136
DOI: 10.1002/glia.22421
ISI #: 000312547400003
Category: A1
Type: Journal Contribution
Validations: ecoom 2014
Appears in Collections:Research publications

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