Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/14537
Title: | Experimental early-life febrile seizures induce changes in GABA(A)R-mediated neurotransmission in the dentate gyrus | Authors: | SWIJSEN, Ann AVILA MACAYA, Ariel BRONE, Bert JANSSEN, Daniel HOOGLAND, Govert RIGO, Jean-Michel |
Issue Date: | 2012 | Publisher: | WILEY-BLACKWELL | Source: | EPILEPSIA, 53 (11), p. 1968-1977 | Abstract: | Purpose: Febrile seizures (FS), the most frequent seizure type during childhood, have been linked to temporal lobe epilepsy (TLE) in adulthood. Yet, underlying mechanisms are still largely unknown. Altered gamma-aminobutyric acid (GABA)ergic neurotransmission in the dentate gyrus (DG) circuit has been hypothesized to be involved. This study aims at analyzing whether experimental FS change inhibitory synaptic input and postsynaptic GABA(A)R function in dentate granule cells. Methods: We applied an immature rat model of hyperthermia (HT)induced FS. GABA(A)R-mediated neurotransmission was studied using whole-cell patch-clamp recordings from dentate granule neurons in hippocampal slices within 69 days post-HT. Key Findings: Frequencies of spontaneous inhibitory postsynaptic currents (sIPSCs) were reduced in HT rats that had experienced seizures, whereas sIPSC amplitudes were enhanced. Whole-cell GABA responses revealed a doubled GABA(A)R sensitivity in dentate granule cells from HT animals, compared to that of normothermic (NT) controls. Analysis of sIPSCs and whole-cell GABA responses showed similar kinetics in postsynaptic GABA(A)Rs of HT and NT rats. quantitative real-time polymerase chain reaction (qPCR) experiments indicated changes in DG GABA(A)R subunit expression, which was most pronounced for the alpha 3 subunit. Significance: The data support the hypothesis that FS persistently alter neuronal excitability. | Notes: | [Swijsen, Ann; Avila, Ariel; Brone, Bert; Janssen, Daniel; Rigo, Jean-Michel] Hasselt Univ Transnat Univ Limburg, BIOMED Res Inst, Diepenbeek, Belgium. [Hoogland, Govert] Univ Med Ctr Maastricht, Sch Mental Hlth & Neurosci, Dept Neurosurg, Maastricht, Netherlands. | Keywords: | Hyperthermia; Hippocampus; Epileptogenesis; gamma-Aminobutyric acid type A receptor; Whole-cell patch-clamp;Clinical Neurology; hyperthermia; hippocampus; epileptogenesis; gamma-aminobutyric acid type A receptor; whole-cell patch-clamp | Document URI: | http://hdl.handle.net/1942/14537 | ISSN: | 0013-9580 | e-ISSN: | 1528-1167 | DOI: | 10.1111/j.1528-1167.2012.03694.x | ISI #: | 000310975400016 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2014 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
swijsen 1.pdf Restricted Access | Published version | 552.29 kB | Adobe PDF | View/Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.