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http://hdl.handle.net/1942/14538
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DC Field | Value | Language |
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dc.contributor.author | BOHRN, Ulrich | - |
dc.contributor.author | Stuetz, E. | - |
dc.contributor.author | Fuchs, K. | - |
dc.contributor.author | Fleischer, M. | - |
dc.contributor.author | Schöning, M.J. | - |
dc.contributor.author | WAGNER, Patrick | - |
dc.date.accessioned | 2013-01-18T13:40:07Z | - |
dc.date.available | 2013-01-18T13:40:07Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | SENSORS AND ACTUATORS B-CHEMICAL, 175, p. 208-217 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | http://hdl.handle.net/1942/14538 | - |
dc.description.abstract | Cell-based sensors for the detection of gases have long been underrepresented, due to the cellular requirement of being cultured in a liquid environment. In this work we established a cell-based gas biosensor for the detection of toxic substances in air, by adapting a commercial sensor chip (Bionas (R)), previously used for the measurement of pollutants in liquids. Cells of the respiratory tract (A549, RPMI 2650, V79), which survive at a gas phase in a natural context, are used as biological receptors. The physiological cell parameters acidification, respiration and morphology are continuously monitored in parallel. Ammonia was used as a highly water-soluble model gas to test the feasibility of the sensor system. Infrared measurements confirmed the sufficiency of the medium draining method. This sensor system provides a basis for many sensor applications such as environmental monitoring, building technology and public security. (C) 2012 Elsevier B.V. All rights reserved. | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.subject.other | Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation; cell-based gas biosensor; whole-cell sensor; A549; RPMI 2650; respiration; acidification; impedance; mid infrared spectroscopy | - |
dc.subject.other | Cell-based gas biosensor; Whole-cell sensor; A549; RPMI 2650; Respiration; Acidification; Impedance; Mid infrared spectroscopy | - |
dc.title | Monitoring of irritant gas using a whole-cell-based sensor system | - |
dc.type | Journal Contribution | - |
local.bibliographicCitation.conferencedate | SEP 04-07, 2011 | - |
local.bibliographicCitation.conferencename | 25th Eurosensors Conference | - |
local.bibliographicCitation.conferenceplace | Athens, GREECE | - |
dc.identifier.epage | 217 | - |
dc.identifier.spage | 208 | - |
dc.identifier.volume | 175 | - |
local.format.pages | 10 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Bohrn, U.; Stuetz, E.; Fuchs, K.; Fleischer, M.] Siemens AG, Corp Res & Technol, D-81739 Munich, Germany. [Bohrn, U.; Schoening, M. J.] Aachen Univ Appl Sci, Inst Nano & Biotechnol, D-52428 Julich, Germany. [Bohrn, U.; Wagner, P.] Univ Hasselt, Inst Mat Res IMO IMOMEC, B-3590 Diepenbeek, Belgium. | - |
local.publisher.place | LAUSANNE | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1016/j.snb.2012.05.088 | - |
dc.identifier.isi | 000312358700036 | - |
item.fulltext | With Fulltext | - |
item.contributor | BOHRN, Ulrich | - |
item.contributor | Stuetz, E. | - |
item.contributor | Fuchs, K. | - |
item.contributor | Fleischer, M. | - |
item.contributor | Schöning, M.J. | - |
item.contributor | WAGNER, Patrick | - |
item.fullcitation | BOHRN, Ulrich; Stuetz, E.; Fuchs, K.; Fleischer, M.; Schöning, M.J. & WAGNER, Patrick (2012) Monitoring of irritant gas using a whole-cell-based sensor system. In: SENSORS AND ACTUATORS B-CHEMICAL, 175, p. 208-217. | - |
item.accessRights | Restricted Access | - |
item.validation | ecoom 2014 | - |
crisitem.journal.eissn | 0925-4005 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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bohrn 1.pdf Restricted Access | Published version | 1.53 MB | Adobe PDF | View/Open Request a copy |
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