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Title: Proteome changes in auricular lymph nodes and serum after dermal sensitization to toluene diisocyanate in mice
Authors: Haenen, Steven
Clynen, Elke 
De Vooght, Vanessa
Schoofs, Liliane
Nemery, Benoit
Hoet, Peter H. M.
Vanoirbeek, Jeroen A. J.
Issue Date: 2012
Source: PROTEOMICS, 12 (23-24), p. 3548-3558
Abstract: Some reactive chemicals, such as diisocyanates, are capable of initiating an allergic response, which can lead to occupational asthma after a latency period. Clinical symptoms such as cough, wheezing, and dyspnea occur only late, making it difficult to intervene at an early stage. So far, most studies using proteomics in lung research have focused on comparisons of healthy versus diseased subjects. Here, using 2D-DIGE, we explored proteome changes in the local draining lymph nodes and serum of mice dermally sensitized once or twice with toluene-2,4-diisocyanate (TDI) before asthma is induced. In the lymph nodes, we found 38 and 58 differentially expressed proteins after one and two treatments, respectively, between TDI-treated and vehicle-treated mice. In serum, seven and 16 differentially expressed proteins were detected after one and two treatments, respectively. We identified 8085% of the differentially expressed proteins by MS. Among them, lymphocyte-specific protein-1, coronin 1a, and hemopexin were verified by Western blotting or ELISA in an independent group of mice. This study revealed alterations in the proteomes early during sensitization in a mouse model before the onset of chemical-induced asthma. If validated in humans, these changes could lead to earlier diagnosis of TDI-exposed workers.
Notes: [Vanoirbeek, Jeroen A. J.] Katholieke Univ Leuven, Dept Publ Hlth Occupat Environm & Insurance Med, B-3000 Louvain, Belgium. [Haenen, Steven; Schoofs, Liliane] Katholieke Univ Leuven, Res Grp Funct Genom & Prote, B-3000 Louvain, Belgium. [Clynen, Elke] Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium.
Keywords: Animal proteomics; Coronin 1a; 2D-DIGE; Isocyanate-induced asthma; Lymphocyte-specific protein-1; Sensitization;Biochemical Research Methods; Biochemistry & Molecular Biology; animal proteomics; coronin 1a; 2D-DIGE; isocyanate-induced asthma; lymphocyte-specific protein-1; sensitization
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ISSN: 1615-9853
e-ISSN: 1615-9861
DOI: 10.1002/pmic.201200264
ISI #: 000312650100011
Category: A1
Type: Journal Contribution
Validations: ecoom 2014
Appears in Collections:Research publications

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