Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/14607
Title: Identification of EPS-degrading activity within the tail spikes of the novel Pseudomonas putida phage AF
Authors: Cornelissen, Anneleen
Ceyssens, Pieter-Jan
Krylov, Victor N.
NOBEN, Jean-Paul 
Volckaert, Guido
Lavigne, Rob
Issue Date: 2012
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Source: VIROLOGY, 434 (2), p. 251-256
Abstract: We report the study of phage AF, the first member of the canonical lambdoid phage group infecting Pseudomonas putida. Its 42.6 kb genome is related to the "epsilon 15-like viruses" and the "BPP-1-like viruses", a clade of bacteriophages shaped by extensive horizontal gene transfer. The AF virions display exopolysaccharide (EPS)-degrading activity, which originates from the action of the C-terminal domain of the tail spike (Gp19). This protein shows high similarity to the tail spike of the T7-like P. putida-infecting phage phi 15. These unrelated phages have an identical host spectrum and EPS degradation characteristics, designating the C-terminal part of Gp19 as sole determinant for these functions. While intact AF particles have biofilm-degrading properties, Gp19 and non-infectious AF particles do not, emphasizing the role of phage amplification in biofilm degradation. (C) 2012 Elsevier Inc. All rights reserved.
Notes: [Cornelissen, Anneleen; Ceyssens, Pieter-Jan; Volckaert, Guido; Lavigne, Rob] Katholieke Univ Leuven, Lab Gene Technol, B-3001 Louvain, Belgium. [Krylov, Victor N.] RAMS, II Mechnikov Res Inst Vaccines & Sera, Lab Bacteriophage Genet, Moscow 105064, Russia. [Noben, Jean-Paul] Hasselt Univ, Biomed Res Inst, Sch Life Sci, B-3590 Diepenbeek, Belgium. [Noben, Jean-Paul] Hasselt Univ, Transnat Univ Limburg, B-3590 Diepenbeek, Belgium.
Keywords: Virology; bacteriophage; P.putida; exopolysaccharide-degrading enzyme; biofilm degradation; horizontal gene transfer;Bacteriophage; P. putida; Exopolysaccharide-degrading enzyme; Biofilm degradation; Horizontal gene transfer
Document URI: http://hdl.handle.net/1942/14607
ISSN: 0042-6822
e-ISSN: 1089-862X
DOI: 10.1016/j.virol.2012.09.030
ISI #: 000312509300014
Category: A1
Type: Journal Contribution
Validations: ecoom 2014
Appears in Collections:Research publications

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