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|Title:||Genome and proteome analysis of 7-7-1, a flagellotropic phage infecting Agrobacterium sp H13-3||Authors:||Kropinski, Andrew M.
Van den Bossche, An
|Issue Date:||2012||Publisher:||BIOMED CENTRAL LTD||Source:||VIROLOGY JOURNAL, 9, Art. No 102||Abstract:||Background: The flagellotropic phage 7-7-1 infects motile cells of Agrobacterium sp H13-3 by attaching to and traveling along the rotating flagellar filament to the secondary receptor at the base, where it injects its DNA into the host cell. Here we describe the complete genomic sequence of 69,391 base pairs of this unusual bacteriophage. Methods: The sequence of the 7-7-1 genome was determined by pyro(454)sequencing to a coverage of 378-fold. It was annotated using MyRAST and a variety of internet resources. The structural proteome was analyzed by SDS-PAGE coupled electrospray ionization-tandem mass spectrometry (MS/MS). Results: Sequence annotation and a structural proteome analysis revealed 127 open reading frames, 84 of which are unique. In six cases 7-7-1 proteins showed sequence similarity to proteins from the virulent Burkholderia myovirus BcepB1A. Unique features of the 7-7-1 genome are the physical separation of the genes encoding the small (orf100) and large (orf112) subunits of the DNA packaging complex and the apparent lack of a holin-lysin cassette. Proteomic analysis revealed the presence of 24 structural proteins, five of which were identified as baseplate (orf7), putative tail fibre (orf102), portal (orf113), major capsid (orf115) and tail sheath (orf126) proteins. In the latter case, the N-terminus was removed during capsid maturation, probably by a putative prohead protease (orf114).||Notes:||[Kropinski, Andrew M.] Publ Hlth Agcy Canada, Lab Foodborne Zoonoses, Guelph, ON N1G 3W4, Canada. [Kropinski, Andrew M.] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada. [Van den Bossche, An; Lavigne, Rob] Katholieke Univ Leuven, Div Gene Technol, B-3001 Heverlee, Belgium. [Noben, Jean-Paul] Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium. [Noben, Jean-Paul] Hasselt Univ, Sch Life Sci, Transnat Univ Limburg, Diepenbeek, Belgium. [Babinger, Patrick] Univ Regensburg, Inst Biophys & Phys Biochem, D-93040 Regensburg, Germany. [Schmitt, Ruediger] Univ Regensburg, Inst Biochem Genet & Mikrobiol, D-93040 Regensburg, Germany.||Keywords:||Agrobacterium; Phage evolution; Phage ecology; Genome; Proteome; Complex flagellum; Bioinformatics; Posttranslational modification;Virology; agrobacterium; phage evolution; phage ecology; genome; proteome; complex flagellum; bioinformatics; posttranslational modification||Document URI:||http://hdl.handle.net/1942/14608||e-ISSN:||1743-422X||DOI:||10.1186/1743-422X-9-102||ISI #:||000312815700001||Category:||A1||Type:||Journal Contribution||Validations:||ecoom 2014|
|Appears in Collections:||Research publications|
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