Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/14608
Title: | Genome and proteome analysis of 7-7-1, a flagellotropic phage infecting Agrobacterium sp H13-3 | Authors: | Kropinski, Andrew M. Van den Bossche, An Lavigne, Rob NOBEN, Jean-Paul Babinger, Patrick Schmitt, Ruediger |
Issue Date: | 2012 | Publisher: | BIOMED CENTRAL LTD | Source: | VIROLOGY JOURNAL, 9, Art. No 102 | Abstract: | Background: The flagellotropic phage 7-7-1 infects motile cells of Agrobacterium sp H13-3 by attaching to and traveling along the rotating flagellar filament to the secondary receptor at the base, where it injects its DNA into the host cell. Here we describe the complete genomic sequence of 69,391 base pairs of this unusual bacteriophage. Methods: The sequence of the 7-7-1 genome was determined by pyro(454)sequencing to a coverage of 378-fold. It was annotated using MyRAST and a variety of internet resources. The structural proteome was analyzed by SDS-PAGE coupled electrospray ionization-tandem mass spectrometry (MS/MS). Results: Sequence annotation and a structural proteome analysis revealed 127 open reading frames, 84 of which are unique. In six cases 7-7-1 proteins showed sequence similarity to proteins from the virulent Burkholderia myovirus BcepB1A. Unique features of the 7-7-1 genome are the physical separation of the genes encoding the small (orf100) and large (orf112) subunits of the DNA packaging complex and the apparent lack of a holin-lysin cassette. Proteomic analysis revealed the presence of 24 structural proteins, five of which were identified as baseplate (orf7), putative tail fibre (orf102), portal (orf113), major capsid (orf115) and tail sheath (orf126) proteins. In the latter case, the N-terminus was removed during capsid maturation, probably by a putative prohead protease (orf114). | Notes: | [Kropinski, Andrew M.] Publ Hlth Agcy Canada, Lab Foodborne Zoonoses, Guelph, ON N1G 3W4, Canada. [Kropinski, Andrew M.] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada. [Van den Bossche, An; Lavigne, Rob] Katholieke Univ Leuven, Div Gene Technol, B-3001 Heverlee, Belgium. [Noben, Jean-Paul] Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium. [Noben, Jean-Paul] Hasselt Univ, Sch Life Sci, Transnat Univ Limburg, Diepenbeek, Belgium. [Babinger, Patrick] Univ Regensburg, Inst Biophys & Phys Biochem, D-93040 Regensburg, Germany. [Schmitt, Ruediger] Univ Regensburg, Inst Biochem Genet & Mikrobiol, D-93040 Regensburg, Germany. | Keywords: | Agrobacterium; Phage evolution; Phage ecology; Genome; Proteome; Complex flagellum; Bioinformatics; Posttranslational modification;Virology; agrobacterium; phage evolution; phage ecology; genome; proteome; complex flagellum; bioinformatics; posttranslational modification | Document URI: | http://hdl.handle.net/1942/14608 | e-ISSN: | 1743-422X | DOI: | 10.1186/1743-422X-9-102 | ISI #: | 000312815700001 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2014 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
genome and proteome analysis.pdf | 998.47 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
12
checked on Sep 2, 2020
WEB OF SCIENCETM
Citations
20
checked on Sep 28, 2024
Page view(s)
76
checked on Apr 26, 2023
Download(s)
130
checked on Apr 26, 2023
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.