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http://hdl.handle.net/1942/14781
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DC Field | Value | Language |
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dc.contributor.author | KEUNEN, Els | - |
dc.contributor.author | REMANS, Tony | - |
dc.contributor.author | OPDENAKKER, Kelly | - |
dc.contributor.author | JOZEFCZAK, Marijke | - |
dc.contributor.author | GIELEN, Heidi | - |
dc.contributor.author | Guisez, Yves | - |
dc.contributor.author | VANGRONSVELD, Jaco | - |
dc.contributor.author | CUYPERS, Ann | - |
dc.date.accessioned | 2013-03-22T10:10:47Z | - |
dc.date.available | 2013-03-22T10:10:47Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | PLANT PHYSIOLOGY AND BIOCHEMISTRY, 63, p. 272-280 | - |
dc.identifier.issn | 0981-9428 | - |
dc.identifier.uri | http://hdl.handle.net/1942/14781 | - |
dc.description.abstract | Lipoxygenases (LOXes, EC 1.13.11.12) are involved in growth, development and responses to stress. Earlier results suggested a role in stress generation, signalling and/or responses when Arabidopsis thaliana is exposed to cadmium (Cd), and expression of the cytosolic LOX1 was highly upregulated in the roots after Cd exposure. To investigate the involvement of LOX1 in early metal stress responses, three-week-old wild-type and lox1-1 mutant A. thaliana plants were acutely (24 h) exposed to realistic Cd concentrations (5 and 10 mu M) and several oxidative stress and signalling related parameters were studied at transcriptional and biochemical levels. Transcription of several genes encoding ROS producing and scavenging enzymes failed to be induced up to wild-type levels after Cd exposure. Expression of 9-LOX enzymes was inhibited in lox1-1 mutant roots due to lack of functional LOX1 and downregulated LOX5 expression, and the lox1-1 mutation also interfered with the expression of genes involved in jasmonate biosynthesis. LOX1 and RBOHD may be involved in stress signalling from roots to shoots, as the induction of APX2 expression, which is dependent on RBOHD activity, was disrupted in lox1-1 while RBOHD failed to be upregulated. A different pattern of H2O2 production and ascorbate and glutathione levels in lox1-1 mutants after Cd exposure may have indirectly influenced gene expression patterns. Although indirect effects of the lox1-1 mutation on gene expression complicate the determination of exact sensing - signalling - response pathways, the results presented here outline a more refined LOX1 functioning in Cd-induced stress responses that could be used in studies determining the exact involvement of LOX1 in these pathways. (c) 2012 Elsevier Masson SAS. All rights reserved. | - |
dc.description.sponsorship | This work was supported by the Research Foundation e Flanders (FWO) by a PhD grant for Els Keunen, a post-doctoral research fellowship for Tony Remans and project [G.0807.09]. Additional funding came from Hasselt University through BOF (Bijzonder Onderzoeksfonds) projects of Hasselt University BOF08G01, BOF07N05 and the Methusalem project (08M03VGRJ). The authors gratefully acknowledge the advice by Professor Dr. R. Carleer for the element determinations. They wish to thank Carine Put and Ann Wijgaerts for skilful technical assistance. The authors are grateful to Danny Huybrecht from the University of Antwerp for HPLC measurements | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER | - |
dc.rights | 2012 Elsevier Masson SAS. All rights reserved. | - |
dc.subject.other | Arabidopsis thaliana | - |
dc.subject.other | Cadmium | - |
dc.subject.other | Metal stress | - |
dc.subject.other | Lipoxy | - |
dc.subject.other | Lipoxygenase | - |
dc.subject.other | Signalling | - |
dc.title | A mutant of the Arabidopsis thaliana LIPOXYGENASE1 gene shows altered signalling and oxidative stress related responses after cadmium exposure | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 280 | - |
dc.identifier.spage | 272 | - |
dc.identifier.volume | 63 | - |
local.format.pages | 9 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Keunen, Els; Remans, Tony; Opdenakker, Kelly; Jozefczak, Marijke; Gielen, Heidi; Vangronsveld, Jaco; Cuypers, Ann] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. [Guisez, Yves] Univ Antwerp, Dept Biol, B-2020 Antwerp, Belgium. | - |
local.publisher.place | P65 RUE CAMILLE DESMOULINS, CS50083, 92442 ISSY-LES-MOULINEAUX, FRANCE | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1016/j.plaphy.2012.12.005 | - |
dc.identifier.isi | 000315011400034 | - |
local.uhasselt.international | no | - |
item.accessRights | Restricted Access | - |
item.fullcitation | KEUNEN, Els; REMANS, Tony; OPDENAKKER, Kelly; JOZEFCZAK, Marijke; GIELEN, Heidi; Guisez, Yves; VANGRONSVELD, Jaco & CUYPERS, Ann (2013) A mutant of the Arabidopsis thaliana LIPOXYGENASE1 gene shows altered signalling and oxidative stress related responses after cadmium exposure. In: PLANT PHYSIOLOGY AND BIOCHEMISTRY, 63, p. 272-280. | - |
item.contributor | KEUNEN, Els | - |
item.contributor | REMANS, Tony | - |
item.contributor | OPDENAKKER, Kelly | - |
item.contributor | JOZEFCZAK, Marijke | - |
item.contributor | GIELEN, Heidi | - |
item.contributor | Guisez, Yves | - |
item.contributor | VANGRONSVELD, Jaco | - |
item.contributor | CUYPERS, Ann | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2014 | - |
crisitem.journal.issn | 0981-9428 | - |
crisitem.journal.eissn | 1873-2690 | - |
Appears in Collections: | Research publications |
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keunen 1.pdf Restricted Access | Published version | 404.77 kB | Adobe PDF | View/Open Request a copy |
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