Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/14782
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dc.contributor.authorAMELOOT, Marcel-
dc.contributor.authorVAN DE VEN, Martin-
dc.contributor.authorUlises Acuna, A.-
dc.contributor.authorValeur, Bernard-
dc.date.accessioned2013-03-22T10:14:55Z-
dc.date.available2013-03-22T10:14:55Z-
dc.date.issued2013-
dc.identifier.citationPURE AND APPLIED CHEMISTRY, 85 (3), p. 589-608-
dc.identifier.issn0033-4545-
dc.identifier.urihttp://hdl.handle.net/1942/14782-
dc.description.abstractAfter recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample properties. A list of selected reference values of steady-state fluorescence anisotropy and polarization is given. Attention is also paid to analysis of time-resolved fluorescence anisotropy data obtained by pulse fluorometry or phase and modulation fluorometry techniques. Recommendations for checking the accuracy of measurements are provided together with a list of selected time-resolved fluorescence anisotropy data as reported in the literature.-
dc.description.sponsorshipThe authors appreciated suggestions made by Dr. Trevor Smith (University of Melbourne). Support by the Spanish Ministry of Science and Innovation 2010CTQ16457 (AUA) and the IAP P6/27 (BELSPO) Functional Supramolecular Systems (MvdV) is thankfully acknowledged. IUPAC Physical and Biophysical Chemistry Division; IUPAC Organic and Biomolecular Chemistry Division; IUPAC Analytical Chemistry Division: see more details on p. 605.-
dc.language.isoen-
dc.publisherINT UNION PURE APPLIED CHEMISTRY-
dc.subject.otherChemistry, Multidisciplinary; anisotropy; fluorescence; IUPAC Analytical Chemistry Division; IUPAC Organic and Biomolecular Chemistry Division; IUPAC Physical and Biophysical Chemistry Division; photoluminescence; polarization; standardization-
dc.subject.otheranisotropy; fluorescence; IUPAC Analytical Chemistry Division; IUPAC Organic and Biomolecular Chemistry Division; IUPAC Physical and Biophysical Chemistry Division; photoluminescence; polarization; standardization-
dc.titleFluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report)-
dc.typeJournal Contribution-
dc.identifier.epage608-
dc.identifier.issue3-
dc.identifier.spage589-
dc.identifier.volume85-
local.format.pages20-
local.bibliographicCitation.jcatA1-
dc.description.notes[Ameloot, Marcel; vandeVen, Martin] Univ Hasselt, BIOMED, B-3590 Diepenbeek, Belgium. [Ulises Acuna, A.] CSIC, Inst Quim Fis Rocasolano, E-28006 Madrid, Spain. [Valeur, Bernard] CNAM, Dept CASER, F-75141 Paris 03, France. [Valeur, Bernard] ENS Cachan, Lab PPSM, F-94235 Cachan, France. marcel.ameloot@uhasselt.be-
local.publisher.placeRES TRIANGLE PK-
local.type.refereedRefereed-
local.type.specifiedArticle-
dc.identifier.doi10.1351/PAC-REP-11-11-12-
dc.identifier.isi000315186600009-
item.fulltextWith Fulltext-
item.contributorAMELOOT, Marcel-
item.contributorVAN DE VEN, Martin-
item.contributorUlises Acuna, A.-
item.contributorValeur, Bernard-
item.fullcitationAMELOOT, Marcel; VAN DE VEN, Martin; Ulises Acuna, A. & Valeur, Bernard (2013) Fluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report). In: PURE AND APPLIED CHEMISTRY, 85 (3), p. 589-608.-
item.accessRightsRestricted Access-
item.validationecoom 2014-
crisitem.journal.issn0033-4545-
crisitem.journal.eissn1365-3075-
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