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Title: Chip-based amperometric enzyme sensor system for monitoring of bioprocesses by flow-injection analysis
Authors: Baecker, M.
Rakowski, D.
Poghossian, A.
Biselli, M.
WAGNER, Patrick 
Schoening, M. J.
Issue Date: 2013
Source: JOURNAL OF BIOTECHNOLOGY, 163 (4), p. 371-376
Abstract: A microfluidic chip integrating amperometric enzyme sensors for the detection of glucose, glutamate and glutamine in cell-culture fermentation processes has been developed. The enzymes glucose oxidase, glutamate oxidase and glutaminase were immobilized by means of cross-linking with glutaraldehyde on platinum thin-film electrodes integrated within a microfluidic channel. The biosensor chip was coupled to a flow-injection analysis system for electrochemical characterization of the sensors. The sensors have been characterized in terms of sensitivity, linear working range and detection limit. The sensitivity evaluated from the respective peak areas was 1.47, 3.68 and 0.28 mu As/mM for the glucose, glutamate and glutamine sensor, respectively. The calibration curves were linear up to a concentration of 20 mM glucose and glutamine and up to 10 mM for glutamate. The lower detection limit amounted to be 0.05 mM for the glucose and glutamate sensor, respectively, and 0.1 mM for the glutamine sensor. Experiments in cell-culture medium have demonstrated a good correlation between the glutamate, glutamine and glucose concentrations measured with the chip-based biosensors in a differential-mode and the commercially available instrumentation. The obtained results demonstrate the feasibility of the realized microfluidic biosensor chip for monitoring of bioprocesses.
Notes: [Baecker, M.; Rakowski, D.; Poghossian, A.; Biselli, M.; Schoening, M. J.] Aachen Univ Appl Sci, INB, Aachen, Germany. [Baecker, M.; Poghossian, A.; Schoening, M. J.] Res Ctr Julich GmbH, PGI 8, Julich, Germany. [Wagner, P.] Hasselt Univ, Inst Mat Res, Diepenbeek, Belgium.
Keywords: Biotechnology & Applied Microbiology; amperometric enzyme sensor; biosensor chip; glucose; glutatate; glutamine; flow-injection analysis;Amperometric enzyme sensor; Biosensor chip; Glucose; Glutamate; Glutamine; Flow-injection analysis
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ISSN: 0168-1656
e-ISSN: 1873-4863
DOI: 10.1016/j.jbiotec.2012.03.014
ISI #: 000315156800004
Category: A1
Type: Journal Contribution
Validations: ecoom 2014
Appears in Collections:Research publications

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