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http://hdl.handle.net/1942/1535
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DC Field | Value | Language |
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dc.contributor.author | GEETS, Joke | - |
dc.contributor.author | Borremans, Brigitte | - |
dc.contributor.author | Diels, Ludo | - |
dc.contributor.author | Springael, Dirk | - |
dc.contributor.author | VANGRONSVELD, Jaco | - |
dc.contributor.author | VAN DER LELIE, Daniel | - |
dc.contributor.author | Vanbroekhoven, Karolien | - |
dc.date.accessioned | 2007-05-31T11:36:51Z | - |
dc.date.available | 2007-05-31T11:36:51Z | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | JOURNAL OF MICROBIOLOGICAL METHODS, 66(2). p. 194-205 | - |
dc.identifier.issn | 0167-7012 | - |
dc.identifier.uri | http://hdl.handle.net/1942/1535 | - |
dc.description.abstract | A denaturing gradient gel electrophoresis (DGGE) method was developed to assess the diversity of dsrB (dissimilatory sulfite reductase β-subunit)-genes in sulfate-reducing communities. For this purpose a PCR primer pair was optimized for the amplification of a similar to 350 bp dsrB gene fragment that after DGGE gel electrophoresis enabled us to discriminate between dsrB genes of different SRB-subgroups,-genera and -species. The dsrB-DGGE method revealed considerable genetic diversity when applied to DNA extracts obtained from aquifer samples that were derived from monitoring wells of an in situ metal precipitation (ISMP) pilot project conducted at the site of a non-ferrous industry or from environmental heavy metal contaminated samples. The sequences of the excised and sequenced DGGE bands represented dsrB genes of different SRB-subgroups,-genera and -species, thus confirming the broad applicability of the PCR primer pair. Linking the results of the physico-chemical follow-up of the field and lab experiments to the dsrB-DGGE data will provide a better understanding of the contribution of the SRB populations to the ongoing ISMP processes. | - |
dc.description.sponsorship | Part of this work was carried out in frame of the 5th framework program of the European Union (Project bMetalbioreduction contract EVK1-CT-1999-00033). J.G. was supported by grants from VITO (Vlaamse Instelling voor Technologisch Onderzoek). D.v.d.L. is being supported by a US Department of Energy Natural and Accelerated Bioremediation Research (NABIR) grant (EE-595-EEDA). | - |
dc.language.iso | en | - |
dc.publisher | Elsevier | - |
dc.rights | (C) 2005 Elsevier B.V. All rights reserved | - |
dc.subject.other | sulfate-reducing bacteria; denaturing gradient gel electrophoresis (DGGE); dissimilatory sulfite reductase (dsr); environmental samples | - |
dc.subject.other | Sulfate-reducing bacteria; Denaturing gradient gel electrophoresis (DGGE); Dissimilatory sulfite reductase (dsr); Environmental samples | - |
dc.title | DsrB gene-based DGGE for community and diversity surveys of sulfate-reducing bacteria | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 205 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 194 | - |
dc.identifier.volume | 66 | - |
local.bibliographicCitation.jcat | A1 | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1016/j.mimet.2005.11.002 | - |
dc.identifier.isi | 000238963000002 | - |
item.contributor | GEETS, Joke | - |
item.contributor | Borremans, Brigitte | - |
item.contributor | Diels, Ludo | - |
item.contributor | Springael, Dirk | - |
item.contributor | VANGRONSVELD, Jaco | - |
item.contributor | VAN DER LELIE, Daniel | - |
item.contributor | Vanbroekhoven, Karolien | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2007 | - |
item.fullcitation | GEETS, Joke; Borremans, Brigitte; Diels, Ludo; Springael, Dirk; VANGRONSVELD, Jaco; VAN DER LELIE, Daniel & Vanbroekhoven, Karolien (2006) DsrB gene-based DGGE for community and diversity surveys of sulfate-reducing bacteria. In: JOURNAL OF MICROBIOLOGICAL METHODS, 66(2). p. 194-205. | - |
item.accessRights | Restricted Access | - |
crisitem.journal.issn | 0167-7012 | - |
crisitem.journal.eissn | 1872-8359 | - |
Appears in Collections: | Research publications |
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1-s2.0-S0167701205003404-main.pdf Restricted Access | Published version | 219.2 kB | Adobe PDF | View/Open Request a copy |
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