Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/15453
Title: Molecularly imprinted polymers as synthetic receptors for the QCM-D-based detection of L-nicotine in diluted saliva and urine samples
Authors: ALENUS, Jan 
ETHIRAJAN, Anitha 
HOREMANS, Frederik 
WEUSTENRAED, Ans 
Csipai, P.
Gruber, J.
PEETERS, Marloes 
CLEIJ, Thomas 
WAGNER, Patrick 
Issue Date: 2013
Publisher: SPRINGER HEIDELBERG
Source: ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 405 (20), p. 6479-6487
Abstract: Molecularly imprinted polymers (MIPs) are synthetic receptors that are able to specifically bind their target molecules in complex samples, making them a versatile tool in biosensor technology. The combination of MIPs as a recognition element with quartz crystal microbalances (QCM-D with dissipation monitoring) gives a straightforward and sensitive device, which can simultaneously measure frequency and dissipation changes. In this work, bulk-polymerized l-nicotine MIPs were used to test the feasibility of l-nicotine detection in saliva and urine samples. First, l-nicotine-spiked saliva and urine were measured after dilution in demineralized water and 0.1x phosphate-buffered saline solution for proof-of-concept purposes. l-nicotine could indeed be detected specifically in the biologically relevant micromolar concentration range. After successfully testing on spiked samples, saliva was analyzed, which was collected during chewing of either nicotine tablets with different concentrations or of smokeless tobacco. The MIPs in combination with QCM-D were able to distinguish clearly between these samples: This proves the functioning of the concept with saliva, which mediates the oral uptake of nicotine as an alternative to the consumption of cigarettes.
Notes: [Alenus, J.; Ethirajan, A.; Horemans, F.; Weustenraed, A.; Csipai, P.; Peeters, M.; Cleij, T. J.; Wagner, P.] Hasselt Univ, Inst Mat Res IMO, B-3590 Diepenbeek, Belgium. [Csipai, P.; Gruber, J.] Univ Sao Paulo, Inst Quim, BR-05508000 Sao Paulo, Brazil. [Peeters, M.; Wagner, P.] IMEC, Div IMOMEC, B-3590 Diepenbeek, Belgium.
Keywords: Biochemical Research Methods; Chemistry, Analytical;Molecularly imprinted polymers; L-nicotine; Nicotine tablets; Smokeless tobacco; Quartz crystal microbalance; Dissipation monitoring
Document URI: http://hdl.handle.net/1942/15453
ISSN: 1618-2642
e-ISSN: 1618-2650
DOI: 10.1007/s00216-013-7080-1
ISI #: 000321911800011
Category: A1
Type: Journal Contribution
Validations: ecoom 2014
Appears in Collections:Research publications

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