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http://hdl.handle.net/1942/15846
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DC Field | Value | Language |
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dc.contributor.author | BERS, Karolien | - |
dc.contributor.author | VAN GRINSVEN, Bart | - |
dc.contributor.author | VANDENRYT, Thijs | - |
dc.contributor.author | MURIB, Mohammed Sharif | - |
dc.contributor.author | JANSSENS, Wim | - |
dc.contributor.author | Geerets, B. | - |
dc.contributor.author | AMELOOT, Marcel | - |
dc.contributor.author | HAENEN, Ken | - |
dc.contributor.author | MICHIELS, Luc | - |
dc.contributor.author | DE CEUNINCK, Ward | - |
dc.contributor.author | WAGNER, Patrick | - |
dc.date.accessioned | 2013-10-23T13:29:39Z | - |
dc.date.available | 2013-10-23T13:29:39Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | DIAMOND AND RELATED MATERIALS, 38, p. 45-51 | - |
dc.identifier.issn | 0925-9635 | - |
dc.identifier.uri | http://hdl.handle.net/1942/15846 | - |
dc.description.abstract | In this article, we report on the label-free real-time thermal monitoring of the denaturation of specific DNA fragments and its potential to detect and quantify single nucleotide polymorphisms (SNPs). Probe DNA, consisting of a 36-mer fragment was covalently immobilized on nanocrystalline chemical vapour deposition (CVD) diamond platforms and hybridized with a 29-mer target DNA fragment (full matching and/or with a point mutation). It was observed that the change in heat transfer resistance upon denaturation is dependent on the amount of DNA hybridized to the nanocrystalline diamond (NCD) surface. Furthermore the possibility to distinguish between a full matching sequence and its singularly mutated counterpart, when bound to the same NCD surface, was investigated. NCD surfaces were selectively hybridized with both full matching and mutated DNA fragments at different ratios (3:1, 2:2 and 1:3). A clear bipartite response in heat transfer resistivity was observed upon simultaneous denaturation of these DNA fragments. Denaturation temperature could be used to identify the DNA fragment to which each partial response could be attributed. Moreover, the partial increases in heat transfer resistivity related to the hybridized amount of non-mutated or mutated DNA, respectively. These results imply that heat transfer resistivity is a technique which can be used to (i) quantify DNA fragments of interest, (ii) detect and (iii) quantify SNPs in a mixture of mutated and non-mutated DNA fragments. Moreover, it illustrates the potential of this technique to detect SNPs without the necessity to design complex microarrays. (c) 2013 Elsevier B.V. All rights reserved. | - |
dc.description.sponsorship | Financial support by the Life-Science Initiative of the Province of Limburg, the Research Foundation Flanders FWO (Project G.0829.09 and project G.0B62.13 N) and the EU FP7 through Marie Curie ITN "MATCON" (PITN-GA-2009-238201) is greatly appreciated. | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.subject.other | CVD diamond; Heat transfer resistance; Mutation analysis; Array | - |
dc.subject.other | Materials Science, Multidisciplinary | - |
dc.title | Implementing heat transfer resistivity as a key element in a nanocrystalline diamond based single nucleotide polymorphism detection array | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 51 | - |
dc.identifier.spage | 45 | - |
dc.identifier.volume | 38 | - |
local.format.pages | 7 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Bers, K.; van Grinsven, B.; Vandenryt, T.; Murib, M.; Janssen, W.; Geerets, B.; Haenen, K.; De Ceuninck, W.; Wagner, P.] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium. [Vandenryt, T.] XIOS Univ Coll, Dept Appl Engn, B-3590 Diepenbeek, Belgium. [Janssen, W.; Haenen, K.; De Ceuninck, W.; Wagner, P.] IMOMEC, B-3590 Diepenbeek, Belgium. [Ameloot, M.] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. karolien.bers@uhasselt.be | - |
local.publisher.place | LAUSANNE | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1016/j.diamond.2013.06.008 | - |
dc.identifier.isi | 000324664700010 | - |
item.contributor | BERS, Karolien | - |
item.contributor | VAN GRINSVEN, Bart | - |
item.contributor | VANDENRYT, Thijs | - |
item.contributor | MURIB, Mohammed Sharif | - |
item.contributor | JANSSENS, Wim | - |
item.contributor | Geerets, B. | - |
item.contributor | AMELOOT, Marcel | - |
item.contributor | HAENEN, Ken | - |
item.contributor | MICHIELS, Luc | - |
item.contributor | DE CEUNINCK, Ward | - |
item.contributor | WAGNER, Patrick | - |
item.fullcitation | BERS, Karolien; VAN GRINSVEN, Bart; VANDENRYT, Thijs; MURIB, Mohammed Sharif; JANSSENS, Wim; Geerets, B.; AMELOOT, Marcel; HAENEN, Ken; MICHIELS, Luc; DE CEUNINCK, Ward & WAGNER, Patrick (2013) Implementing heat transfer resistivity as a key element in a nanocrystalline diamond based single nucleotide polymorphism detection array. In: DIAMOND AND RELATED MATERIALS, 38, p. 45-51. | - |
item.accessRights | Restricted Access | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2014 | - |
crisitem.journal.issn | 0925-9635 | - |
crisitem.journal.eissn | 1879-0062 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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bers 1.pdf Restricted Access | Published version | 1.3 MB | Adobe PDF | View/Open Request a copy |
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