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http://hdl.handle.net/1942/15954
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DC Field | Value | Language |
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dc.contributor.author | Rosenow, Anja | - |
dc.contributor.author | NOBEN, Jean-Paul | - |
dc.contributor.author | Bouwman, Freek | - |
dc.contributor.author | MARIMAN, Edwin | - |
dc.contributor.author | Renes, Johan | - |
dc.date.accessioned | 2013-11-07T08:40:30Z | - |
dc.date.available | 2013-11-07T08:40:30Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1834 (12), p. 2761-2771 | - |
dc.identifier.issn | 1570-9639 | - |
dc.identifier.uri | http://hdl.handle.net/1942/15954 | - |
dc.description.abstract | White adipose tissue (WAT) regulates energy metabolismby secretion of proteins with endocrine and paracrine effects. Dysregulation of the secretome of obesity-associated enlarged WAT may lead to obesity related disorders. This can be caused by hypoxia as a result of poorly vascularized WAT. The effect of hypoxia on the secretome of human (pre)adipocytes is largely unknown. Therefore,we investigated the effect of CoCl2, a hypoxia mimetic, on the secretome of human SGBS (pre)adipocytes by a proteomics approach combined with bioinformatic analysis. In addition, regulation of protein secretion was examined by protein turnover experiments. As such, secretome changes were particularly associated with protein down-regulation and extracellular matrix protein dysregulation. The observed up-regulation of collagens in adipocytes may be essential for cell survival while down-regulation of collagens in preadipocytes may indicate a disturbed differentiation process. These CoCl2-induced changes reflect WAT dysfunction that ultimately may lead to obesity-associated complications. In addition, 9 novel adipocyte secreted proteins were identified from which 6were regulated by CoCl2.Mass spectrometry data have been deposited to the ProteomeXchange with identifier PXD000162. © 2013 Elsevier B.V. All rights reserved. | - |
dc.description.sponsorship | This study was supported by a grant from the School of Life Sciences, transnationale Universiteit Limburg to JR. Dr. Martin Wabitsch (Ulm University Medical Center, Germany) is kindly acknowledged for providing SGBS cells, Eric Royackers (University Hasselt, Belgium) for practical support and Dr. Johan Jocken (Maastricht University, The Netherlands) for helpful discussions. The PRIDE team is acknowledged for handling our MS data. This study is MIAPE compliant | - |
dc.language.iso | en | - |
dc.subject.other | Human (pre)adipocyte; Hypoxia-mimetic; Secretome; Turnover | - |
dc.title | Hypoxia-mimetic Effects in the Secretome of Human Preadipocytes and Adipocytes. | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 2771 | - |
dc.identifier.issue | 12 | - |
dc.identifier.spage | 2761 | - |
dc.identifier.volume | 1834 | - |
local.bibliographicCitation.jcat | A1 | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1016/j.bbapap.2013.10.003 | - |
dc.identifier.isi | 000329418300034 | - |
item.contributor | Rosenow, Anja | - |
item.contributor | NOBEN, Jean-Paul | - |
item.contributor | Bouwman, Freek | - |
item.contributor | MARIMAN, Edwin | - |
item.contributor | Renes, Johan | - |
item.validation | ecoom 2015 | - |
item.fulltext | With Fulltext | - |
item.accessRights | Restricted Access | - |
item.fullcitation | Rosenow, Anja; NOBEN, Jean-Paul; Bouwman, Freek; MARIMAN, Edwin & Renes, Johan (2013) Hypoxia-mimetic Effects in the Secretome of Human Preadipocytes and Adipocytes.. In: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1834 (12), p. 2761-2771. | - |
crisitem.journal.issn | 1570-9639 | - |
crisitem.journal.eissn | 1878-1454 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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hypoxia.pdf Restricted Access | Published version | 1.24 MB | Adobe PDF | View/Open Request a copy |
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