Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16030
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dc.contributor.authorBustin, Stephen A.-
dc.contributor.authorBenes, Vladimir-
dc.contributor.authorGarson, Jeremy-
dc.contributor.authorHellemans, Jan-
dc.contributor.authorHuggett, Jim-
dc.contributor.authorKubista, Mikael-
dc.contributor.authorMueller, Reinhold-
dc.contributor.authorNolan, Tania-
dc.contributor.authorPfaffl, Michael W.-
dc.contributor.authorShipley, Gregory-
dc.contributor.authorWittwer, Carl T.-
dc.contributor.authorSchjerling, Peter-
dc.contributor.authorDay, Philip J.-
dc.contributor.authorAbreu, Monica-
dc.contributor.authorAguado, Begona-
dc.contributor.authorBeaulieu, Jean-Francois-
dc.contributor.authorBeckers, Anneleen-
dc.contributor.authorBogaert, Sara-
dc.contributor.authorBrowne, John A.-
dc.contributor.authorCarrasco-Ramiro, Fernando-
dc.contributor.authorCeelen, Liesbeth-
dc.contributor.authorCiborowski, Kate-
dc.contributor.authorCornillie, Pieter-
dc.contributor.authorCoulon, Stephanie-
dc.contributor.authorCUYPERS, Ann-
dc.contributor.authorDe Brouwer, Sara-
dc.contributor.authorDe Ceuninck, Leentje-
dc.contributor.authorDe Craene, Jurgen-
dc.contributor.authorDe Naeyer, Helene-
dc.contributor.authorDe Spiegelaere, Ward-
dc.contributor.authorDeckers, Kato-
dc.contributor.authorDheedene, Annelies-
dc.contributor.authorDurinck, Kaat-
dc.contributor.authorFerreira-Teixeira, Margarida-
dc.contributor.authorFieuw, Annelies-
dc.contributor.authorGallup, Jack M.-
dc.contributor.authorGonzalo-Flores, Sandra-
dc.contributor.authorGoossens, Karen-
dc.contributor.authorHeindryckx, Femke-
dc.contributor.authorHerring, Elizabeth-
dc.contributor.authorHoenicka, Hans-
dc.contributor.authorIcardi, Laura-
dc.contributor.authorJaggi, Rolf-
dc.contributor.authorJavad, Farzad-
dc.contributor.authorKarampelias, Michael-
dc.contributor.authorKibenge, Frederick-
dc.contributor.authorKibenge, Molly-
dc.contributor.authorKumps, Candy-
dc.contributor.authorLambertz, Irina-
dc.contributor.authorLammens, Tim-
dc.contributor.authorMarkey, Amelia-
dc.contributor.authorMESSIAEN, Peter-
dc.contributor.authorMets, Evelien-
dc.contributor.authorMorais, Sofia-
dc.contributor.authorMudarra-Rubio, Alberto-
dc.contributor.authorNakiwala, Justine-
dc.contributor.authorNelis, Hilde-
dc.contributor.authorOlsvik, Pal A.-
dc.contributor.authorPerez-Novo, Claudina-
dc.contributor.authorPLUSQUIN, Michelle-
dc.contributor.authorREMANS, Tony-
dc.contributor.authorRihani, Ali-
dc.contributor.authorRodrigues-Santos, Paulo-
dc.contributor.authorRondou, Pieter-
dc.contributor.authorSanders, Rebecca-
dc.contributor.authorSchmidt-Bleek, Katharina-
dc.contributor.authorSkovgaard, Kerstin-
dc.contributor.authorSMEETS, Karen-
dc.contributor.authorTabera, Laura-
dc.contributor.authorToegel, Stefan-
dc.contributor.authorVan Acker, Tim-
dc.contributor.authorVan den Broeck, Wim-
dc.contributor.authorVan der Meulen, Joni-
dc.contributor.authorVan Gele, Mireille-
dc.contributor.authorVan Peer, Gert-
dc.contributor.authorVan Poucke, Mario-
dc.contributor.authorVan Roy, Nadine-
dc.contributor.authorVergult, Sarah-
dc.contributor.authorWauman, Joris-
dc.contributor.authorTshuikina-Wiklander, Marina-
dc.contributor.authorWillems, Erik-
dc.contributor.authorZaccara, Sara-
dc.contributor.authorZeka, Fjoralba-
dc.contributor.authorVandesompele, Jo-
dc.date.accessioned2013-12-04T10:20:18Z-
dc.date.available2013-12-04T10:20:18Z-
dc.date.issued2013-
dc.identifier.citationNATURE METHODS, 10 (11), p. 1063-1067-
dc.identifier.issn1548-7091-
dc.identifier.urihttp://hdl.handle.net/1942/16030-
dc.description.abstractTwo surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.-
dc.language.isoen-
dc.titleThe need for transparency and good practices in the qPCR literature-
dc.typeJournal Contribution-
dc.identifier.epage1067-
dc.identifier.issue11-
dc.identifier.spage1063-
dc.identifier.volume10-
local.bibliographicCitation.jcatA1-
dc.description.notesBustin, SA (reprint author), Anglia Ruskin Univ, Postgrad Med Inst, Chelmsford, Essex, England. stephen.bustin@anglia.ac.uk-
local.type.refereedRefereed-
local.type.specifiedArticle-
dc.identifier.doi10.1038/nmeth.2697-
dc.identifier.isi000326507600016-
item.accessRightsClosed Access-
item.fulltextNo Fulltext-
item.fullcitationBustin, Stephen A.; Benes, Vladimir; Garson, Jeremy; Hellemans, Jan; Huggett, Jim; Kubista, Mikael; Mueller, Reinhold; Nolan, Tania; Pfaffl, Michael W.; Shipley, Gregory; Wittwer, Carl T.; Schjerling, Peter; Day, Philip J.; Abreu, Monica; Aguado, Begona; Beaulieu, Jean-Francois; Beckers, Anneleen; Bogaert, Sara; Browne, John A.; Carrasco-Ramiro, Fernando; Ceelen, Liesbeth; Ciborowski, Kate; Cornillie, Pieter; Coulon, Stephanie; CUYPERS, Ann; De Brouwer, Sara; De Ceuninck, Leentje; De Craene, Jurgen; De Naeyer, Helene; De Spiegelaere, Ward; Deckers, Kato; Dheedene, Annelies; Durinck, Kaat; Ferreira-Teixeira, Margarida; Fieuw, Annelies; Gallup, Jack M.; Gonzalo-Flores, Sandra; Goossens, Karen; Heindryckx, Femke; Herring, Elizabeth; Hoenicka, Hans; Icardi, Laura; Jaggi, Rolf; Javad, Farzad; Karampelias, Michael; Kibenge, Frederick; Kibenge, Molly; Kumps, Candy; Lambertz, Irina; Lammens, Tim; Markey, Amelia; MESSIAEN, Peter; Mets, Evelien; Morais, Sofia; Mudarra-Rubio, Alberto; Nakiwala, Justine; Nelis, Hilde; Olsvik, Pal A.; Perez-Novo, Claudina; PLUSQUIN, Michelle; REMANS, Tony; Rihani, Ali; Rodrigues-Santos, Paulo; Rondou, Pieter; Sanders, Rebecca; Schmidt-Bleek, Katharina; Skovgaard, Kerstin; SMEETS, Karen; Tabera, Laura; Toegel, Stefan; Van Acker, Tim; Van den Broeck, Wim; Van der Meulen, Joni; Van Gele, Mireille; Van Peer, Gert; Van Poucke, Mario; Van Roy, Nadine; Vergult, Sarah; Wauman, Joris; Tshuikina-Wiklander, Marina; Willems, Erik; Zaccara, Sara; Zeka, Fjoralba & Vandesompele, Jo (2013) The need for transparency and good practices in the qPCR literature. In: NATURE METHODS, 10 (11), p. 1063-1067.-
item.validationecoom 2015-
item.contributorBustin, Stephen A.-
item.contributorBenes, Vladimir-
item.contributorGarson, Jeremy-
item.contributorHellemans, Jan-
item.contributorHuggett, Jim-
item.contributorKubista, Mikael-
item.contributorMueller, Reinhold-
item.contributorNolan, Tania-
item.contributorPfaffl, Michael W.-
item.contributorShipley, Gregory-
item.contributorWittwer, Carl T.-
item.contributorSchjerling, Peter-
item.contributorDay, Philip J.-
item.contributorAbreu, Monica-
item.contributorAguado, Begona-
item.contributorBeaulieu, Jean-Francois-
item.contributorBeckers, Anneleen-
item.contributorBogaert, Sara-
item.contributorBrowne, John A.-
item.contributorCarrasco-Ramiro, Fernando-
item.contributorCeelen, Liesbeth-
item.contributorCiborowski, Kate-
item.contributorCornillie, Pieter-
item.contributorCoulon, Stephanie-
item.contributorCUYPERS, Ann-
item.contributorDe Brouwer, Sara-
item.contributorDe Ceuninck, Leentje-
item.contributorDe Craene, Jurgen-
item.contributorDe Naeyer, Helene-
item.contributorDe Spiegelaere, Ward-
item.contributorDeckers, Kato-
item.contributorDheedene, Annelies-
item.contributorDurinck, Kaat-
item.contributorFerreira-Teixeira, Margarida-
item.contributorFieuw, Annelies-
item.contributorGallup, Jack M.-
item.contributorGonzalo-Flores, Sandra-
item.contributorGoossens, Karen-
item.contributorHeindryckx, Femke-
item.contributorHerring, Elizabeth-
item.contributorHoenicka, Hans-
item.contributorIcardi, Laura-
item.contributorJaggi, Rolf-
item.contributorJavad, Farzad-
item.contributorKarampelias, Michael-
item.contributorKibenge, Frederick-
item.contributorKibenge, Molly-
item.contributorKumps, Candy-
item.contributorLambertz, Irina-
item.contributorLammens, Tim-
item.contributorMarkey, Amelia-
item.contributorMESSIAEN, Peter-
item.contributorMets, Evelien-
item.contributorMorais, Sofia-
item.contributorMudarra-Rubio, Alberto-
item.contributorNakiwala, Justine-
item.contributorNelis, Hilde-
item.contributorOlsvik, Pal A.-
item.contributorPerez-Novo, Claudina-
item.contributorPLUSQUIN, Michelle-
item.contributorREMANS, Tony-
item.contributorRihani, Ali-
item.contributorRodrigues-Santos, Paulo-
item.contributorRondou, Pieter-
item.contributorSanders, Rebecca-
item.contributorSchmidt-Bleek, Katharina-
item.contributorSkovgaard, Kerstin-
item.contributorSMEETS, Karen-
item.contributorTabera, Laura-
item.contributorToegel, Stefan-
item.contributorVan Acker, Tim-
item.contributorVan den Broeck, Wim-
item.contributorVan der Meulen, Joni-
item.contributorVan Gele, Mireille-
item.contributorVan Peer, Gert-
item.contributorVan Poucke, Mario-
item.contributorVan Roy, Nadine-
item.contributorVergult, Sarah-
item.contributorWauman, Joris-
item.contributorTshuikina-Wiklander, Marina-
item.contributorWillems, Erik-
item.contributorZaccara, Sara-
item.contributorZeka, Fjoralba-
item.contributorVandesompele, Jo-
crisitem.journal.issn1548-7091-
crisitem.journal.eissn1548-7105-
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