Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/1603
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dc.contributor.authorPérez-Bueno, M.-
dc.contributor.authorCISCATO, Massimo-
dc.contributor.authorVAN DE VEN, Martin-
dc.contributor.authorGarcia-Luque, I.-
dc.contributor.authorVALCKE, Roland-
dc.contributor.authorBarón, M.-
dc.date.accessioned2007-06-07T12:09:26Z-
dc.date.available2007-06-07T12:09:26Z-
dc.date.issued2006-
dc.identifier.citationPHOTOSYNTHESIS RESEARCH, 90(2). p. 111-123-
dc.identifier.issn0166-8595-
dc.identifier.urihttp://hdl.handle.net/1942/1603-
dc.description.abstractWe have studied by kinetic Chl-fluorescence imaging (Chl-FI) Nicotiana benthamiana plants infected with the Italian strain of the pepper mild mottle tobamovirus (PMMoV-I). We have mapped leaf photosynthesis at different points of the fluorescence induction curve as well as at different post-infection times. Images of different fluorescence parameters were obtained to investigate which one could discriminate control from infected leaves in the absence of symptoms. The non-photochemical quenching (NPQ) of excess energy in photosystem II (PSII) seems to be the most adequate chlorophyll fluorescence parameter to assess the effect of tobamoviral infection on the chloroplast. Non-symptomatic mature leaves from inoculated plants displayed a very characteristic time-varying NPQ pattern. In addition, a correlation between NPQ amplification and virus localization by tissue-print was found, suggesting that an increase in the local NPQ values is associated with the areas invaded by the pathogen. Changes in chloroplast ultrastructure in non-symptomatic leaf areas showing different NPQ levels were also investigated. A gradient of ultrastructural modifications was observed among the different areas.-
dc.language.isoen-
dc.publisherSpringer-
dc.subject.otherBiotic stress; Chlorophyll fluorescence imaging; Non-photochemical quenching; Pepper mild mottle virus; Photosystem II; Tobamovirus-
dc.titleImaging viral infection: studies on Nicotiana benthamiana plants infected with the pepper mild mottle tobamovirus-
dc.typeJournal Contribution-
dc.identifier.epage123-
dc.identifier.issue2-
dc.identifier.spage111-
dc.identifier.volume90-
local.bibliographicCitation.jcatA1-
local.type.refereedRefereed-
local.type.specifiedArticle-
dc.bibliographicCitation.oldjcatA1-
dc.identifier.doi10.1007/s11120-006-9098-0-
dc.identifier.isi000243623900003-
item.fulltextNo Fulltext-
item.validationecoom 2008-
item.contributorVALCKE, Roland-
item.contributorBarón, M.-
item.contributorPérez-Bueno, M.-
item.contributorGarcia-Luque, I.-
item.contributorVAN DE VEN, Martin-
item.contributorCISCATO, Massimo-
item.fullcitationPérez-Bueno, M.; CISCATO, Massimo; VAN DE VEN, Martin; Garcia-Luque, I.; VALCKE, Roland & Barón, M. (2006) Imaging viral infection: studies on Nicotiana benthamiana plants infected with the pepper mild mottle tobamovirus. In: PHOTOSYNTHESIS RESEARCH, 90(2). p. 111-123.-
item.accessRightsClosed Access-
crisitem.journal.issn0166-8595-
crisitem.journal.eissn1573-5079-
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